Identification of the cDNA of differentially expressed genes in the transition zone of cucumber seedlings by fluorescent differential display.

Minobu Shimizu, Keita Suzuki, Nobuharu Fujii, Hideyuki Takahashi

Research output: Contribution to journalArticlepeer-review

Abstract

Cucumber seedlings have potential to develop two pegs on the transition zone between the hypocotyl and root. Seedlings grown in a horizontal position suppress the development of the peg on the upper side of the transition zone in response to gravity. To understand how the response to gravity suppresses peg formation, we screened cucumber mRNAs to identify the mRNA in the non-peg side that accumulates more than in the peg side. For screening, we determined conditions of fluorescent differential display (FDD). Then, we carried out FDD and found 4 cDNA bands that repeatedly showed stronger intensity in the non-peg side than the peg side. We isolated one of these RT-PCR products. Northern blotting showed the pattern of its mRNA accumulations corresponding to the results of FDD.

Original languageEnglish
Pages (from-to)232-233
Number of pages2
JournalBiological sciences in space = Uchū seibutsu kagaku
Volume17
Issue number3
Publication statusPublished - 2003 Oct

ASJC Scopus subject areas

  • Medicine(all)

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