Identification of the bphA and bphB Genes of Pseudomonas sp. Strain KKS102 Involved in degradation of biphenyl and polychlorinated biphenyls

Masao Fukuda, Yuji Yasukochi, Yutaka Kikuchi, Yuji Nagata, Kazuhide Kimbara, Hiroyuki Horiuchi, Masamichi Takagi, Keiji Yano

Research output: Contribution to journalArticlepeer-review

67 Citations (Scopus)

Abstract

The nucleotide sequence of the upstream region of the bphC gene from Pseudomonas sp. strain KKS102 was determined. Four genes were found in this region. Deduced amino acid sequences of the first, second, third and fourth genes showed significant homology with a large subunit of iron-sulfur protein, a small subunit of iron-sulfur protein, ferredoxin and dihydrodiol dehydrogenase, respectively, from other bacteria which degrade biphenyl/polychlorinated biphenyls, toluene and benzene. E. coli, in which the four genes, bphC and the gene for ferredoxin reductase from benzene degrading bacterium were expressed, was able to produce meta-cleavage compounds from chlorinated biphenyls. These results show that these gene products are functional in both biphenyl and polychlorinated biphenyls degradation.

Original languageEnglish
Pages (from-to)850-856
Number of pages7
JournalBiochemical and biophysical research communications
Volume202
Issue number2
DOIs
Publication statusPublished - 1994 Jul 29
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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