Identification of the antigenic determinants of factors 8, 9, and 34 of genus Candida

Hidemitsu Kobayashi, Hiroko Oyamada, Akifumi Suzuki, Nobuyuki Shibata, Shigeo Suzuki, Yoshio Okawa

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

We investigated the antigenic determinants of factors 8, 9, and 34 of the genus Candida among pathogenic yeasts by enzyme-linked immunosorbent assay (ELISA) using mannans of Saccharomyces cerevisiae wild type and mutant types, mnn 1-mnn 4 and mnn 2. Results of ELISA including antisera against the antigenic factors of genus Candida (Candida Check, Iatron; FAbs) indicated that these three types of mannan distinctly react with FAbs 34, 8 and 9, respectively. To identify the recognition sites of these FAbs, we compared the ability of various oligosaccharides to inhibit the binding of the mannans to FAbs. The results indicated that FAb 34 preferentially recognizes linear side chains containing a non-reducing terminal α-1,3-linked mannose residue, Manα1 → 3Manα1 → (2Manα1 →)(n)2Man (n ≤ 0), and that one of the recognition sites of FAb 9 is linear α-1,6-linked oligomannosyl series, Manα1 → (6Manα1 →)(n)6-Man (n ≤ 2). On the other hand, the recognition site of FAb 8 apparently consisted of two α-1,2-linked oligomannosyl side chains and an α-1,6-linked mannose residue that originated from the mannan backbone, Manα1 → 2Manα1 → 2(Manα1 → 2-Manα1 → 6)Man.

Original languageEnglish
Pages (from-to)109-112
Number of pages4
JournalFEBS Letters
Volume395
Issue number2-3
DOIs
Publication statusPublished - 1996 Oct 21

Keywords

  • Antigenic factor
  • Cell wall mannan
  • Enzyme-linked immunosorbent assay
  • Pathogenic genus Candida
  • Saccharomyces cerevisae

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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