Identification of six major outer membrane proteins from Actinobacillus actinomycetemcomitans

Hitoshi Komatsuzawa, Ryuji Asakawa, Toshihisa Kawai, Kyoko Ochiai, Tamaki Fujiwara, Martin A. Taubman, Masaru Ohara, Hidemi Kurihara, Motoyuki Sugai

Research output: Contribution to journalArticlepeer-review

46 Citations (Scopus)

Abstract

We have identified six major sarcosyl-insoluble outer membrane proteins (Omp) of Actinobacillus actinomycetemcomitans Y4, and designated them as Omp100, Omp64, Omp39, Omp29, Omp18 and Omp16 according to the molecular mass. A similar N-terminal sequence was found in the first 15 amino acid residues of Omp16 and Omp18. The N-terminal sequence of Omp29 matched perfectly with the sequence previously identified. We cloned and determined the DNA sequences of three complete genes encoding Omp100, Omp64 and Omp18/16, and one incomplete gene encoding Omp39. Each Omp revealed homologies with some bacterial virulence factors responsible for adhesion, invasion, serum resistance, or protein antigenicity. Serum from patients with periodontitis suspected to be related to A. actinomycetemcomintans infection strongly reacted with Omp100, Omp29 and Omp16 as did serum from mice immunized with A. actinomycetemcomitans Y4 whole bacteria. These findings suggest that Omps of A. actinomycetemcomitans can be associated with periodontal disease.

Original languageEnglish
Pages (from-to)195-201
Number of pages7
JournalGene
Volume288
Issue number1-2
DOIs
Publication statusPublished - 2002 Apr 17

Keywords

  • Omp29
  • Periodontitis
  • Serum
  • Virulence

ASJC Scopus subject areas

  • Genetics

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