Identification of selenoprotein P fragments as a cell-death inhibitory factor

Masaki Hirashima, Takeshi Naruse, Hiroaki Maeda, Chikateru Nozaki, Yoshiro Saito, Kazuhiko Takahashi

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

Megakaryoblastoma (Dami cells) cultured in a serum-free medium containing albumin, proliferated for three days but died on the fourth day. This cell death was not observed when human plasma was added, suggesting that human plasma contains a cell-death inhibitory factor. In order to identify this factor, we purified it from human plasma. N-terminal amino acid sequence analysis revealed that this factor is a mixture of C-terminal fragments of selenoprotein P, a major selenocysteine-containing protein in plasma. The specific activity (unit per pmol of selenium) of selenoprotein P fragments protein was 15-fold and 1900-fold higher than that of the full-length SeP and sodium selenite, respectively.

Original languageEnglish
Pages (from-to)794-798
Number of pages5
JournalBiological and Pharmaceutical Bulletin
Volume26
Issue number6
DOIs
Publication statusPublished - 2003 Jun
Externally publishedYes

Keywords

  • Cell death
  • Selenium
  • Selenoprotein P
  • Serum-free culture, albumin

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

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