Abstract
Megakaryoblastoma (Dami cells) cultured in a serum-free medium containing albumin, proliferated for three days but died on the fourth day. This cell death was not observed when human plasma was added, suggesting that human plasma contains a cell-death inhibitory factor. In order to identify this factor, we purified it from human plasma. N-terminal amino acid sequence analysis revealed that this factor is a mixture of C-terminal fragments of selenoprotein P, a major selenocysteine-containing protein in plasma. The specific activity (unit per pmol of selenium) of selenoprotein P fragments protein was 15-fold and 1900-fold higher than that of the full-length SeP and sodium selenite, respectively.
Original language | English |
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Pages (from-to) | 794-798 |
Number of pages | 5 |
Journal | Biological and Pharmaceutical Bulletin |
Volume | 26 |
Issue number | 6 |
DOIs | |
Publication status | Published - 2003 Jun |
Externally published | Yes |
Keywords
- Cell death
- Selenium
- Selenoprotein P
- Serum-free culture, albumin
ASJC Scopus subject areas
- Pharmacology
- Pharmaceutical Science