Identification of SATB1 as a specific biomarker for lymph node metastasis in colorectal cancer

Hironobu Baba, Toshiaki Ishikawa, Kaoru Mogushi, Megumi Ishiguro, Hiroyuki Uetake, Hiroshi Tanaka, Kenichi Sugihara

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)


Background/Aim: The integration of gene expression analysis and genomic profiling represents an efficient approach to the discovery of cancer-related genes. Lymph node metastasis (LNM) is a significant prognostic factor in colorectal cancer (CRC). Detection and analysis of factors related to LNM will help develop new diagnostic methods or therapies. In this study, we aimed to identify genes that are significantly related to LNM in CRC through integrated copy number analysis (CNA) and gene expression analysis. Materials and Methods: Genes showing both upregulated expression and copy number gains in cases involving CRC with LNM were extracted as candidate biomarkers. Expression of the mRNA of the final candidate was validated in 124 patients using quantitative reverse transcription polymerase chain reaction (qRT-PCR) assays. Expression of the protein encoded by this candidate gene was assessed using immunohistochemical (IHC) staining of tissue from 328 patients. The association between protein expression and clinicopathological features was also examined. Results: Special AT-rich sequence-binding protein 1 (SATB1) was extracted from integrated microarray analysis. SATB1 mRNA expression in cancer tissue was significantly higher in patients with LNM than without LNM. SATB1 protein overexpression was significantly associated with LNM. Moreover, overexpression of SATB1 was an independent poor prognostic factor in stage I-III, especially in stage II CRC. Conclusion: SATB1 may play an important role in LNM of CRC. SATB1 may be a biomarker of LNM and of recurrence after surgery for CRC.

Original languageEnglish
Pages (from-to)4069-4072
Number of pages4
JournalAnticancer research
Issue number8
Publication statusPublished - 2016 Aug


  • Biomarker
  • Colorectal cancer
  • Microarray analysis
  • Prognostic factor
  • Special AT-rich sequence-binding protein 1 (SATB1)

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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