Identification of regions responsible for the function of the plant K+ channels KAT1 and AKT2 in Saccharomyces cerevisiae and Xenopus laevis oocytes

Shunya Saito, Naomi Hoshi, Lalu Zulkifli, Sri Widyastuti, Shinobu Goshima, Ingo Dreyer, Nobuyuki Uozumi

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The Arabidopsis K+ channel KAT1 complements in K+-limited medium the growth of the K+ uptake defective Saccharomyces cerevisiae mutant strain CY162, while another K+ channel, AKT2, does not. To gain insight into the structural basis for this difference, we constructed 12 recombinant chimeric channels from these two genes. When expressed in CY162, only three of these chimeras fully rescued the growth of CY162 under K+-limited conditions. We conclude that the transmembrane core region of KAT1 is important for its activity in S. cerevisiae. This involves not only the pore region but also parts of its voltage-sensor domain.

Original languageEnglish
Pages (from-to)510-516
Number of pages7
JournalChannels
Volume11
Issue number6
DOIs
Publication statusPublished - 2017 Nov 2

Keywords

  • AKT2
  • KAT1
  • membrane transport
  • potassium channels
  • transporter

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

Fingerprint Dive into the research topics of 'Identification of regions responsible for the function of the plant K<sup>+</sup> channels KAT1 and AKT2 in Saccharomyces cerevisiae and Xenopus laevis oocytes'. Together they form a unique fingerprint.

  • Cite this