TY - JOUR
T1 - Identification of regions in which positive selection may operate in S-RNase of Rosaceae
T2 - Implication for S-allele-specific recognition sites in S-RNase
AU - Ishimizu, Takeshi
AU - Endo, Toshinori
AU - Yamaguchi-Kabata, Yumi
AU - Nakamura, Kazuo T.
AU - Sakiyama, Fumio
AU - Norioka, Shigemi
N1 - Funding Information:
We are grateful to Ken Nishikawa and Motonori Ota (National Institute of Genetics, Japan) for predicting the tertiary structures of the S-RNases by the 3D-1D method and valuable suggestions. This work was supported in part by a Grant-in-Aid for Scientific Research on a Priority Area (Genetic Dissection of Sexual Differentiation and Pollination Process in Higher Plants, No. 07281103) from the Ministry of Education, Science, Sports and Culture of Japan and by Research Fellowships for Young Scientists provided by the Japan Society for the Promotion of Science.
PY - 1998/12/4
Y1 - 1998/12/4
N2 - A stylar S-RNase is associated with gametophytic self-incompatibility in the Rosaceae, Solanaceae, and Scrophulariaceae. This S-RNase is responsible for S-allele-specific recognition in the self-incompatible reaction, but how it functions in specific discrimination is not clear. Window analysis of the numbers of synonymous (d(S)) and non-synonymous (d(N)) substitutions in rosaceous S-RNases detected four regions with an excess of d(N) over d(S) in which positive selection may operate (PS regions). The topology of the secondary structure of the S-RNases predicted by the PHD method is very similar to that of fungal RNase Rh whose tertiary structure is known. When the sequences of S-RNases are aligned with the sequence of RNase Rh based on the predicted secondary structures, the four PS regions correspond to two surface sites on the tertiary structure of RNase Rh. These findings suggest that in S-RNases the PS regions also form two sites and are candidates for the recognition sites for S-allele-specific discrimination. Copyright (C) 1998 Federation of European Biochemical Societies.
AB - A stylar S-RNase is associated with gametophytic self-incompatibility in the Rosaceae, Solanaceae, and Scrophulariaceae. This S-RNase is responsible for S-allele-specific recognition in the self-incompatible reaction, but how it functions in specific discrimination is not clear. Window analysis of the numbers of synonymous (d(S)) and non-synonymous (d(N)) substitutions in rosaceous S-RNases detected four regions with an excess of d(N) over d(S) in which positive selection may operate (PS regions). The topology of the secondary structure of the S-RNases predicted by the PHD method is very similar to that of fungal RNase Rh whose tertiary structure is known. When the sequences of S-RNases are aligned with the sequence of RNase Rh based on the predicted secondary structures, the four PS regions correspond to two surface sites on the tertiary structure of RNase Rh. These findings suggest that in S-RNases the PS regions also form two sites and are candidates for the recognition sites for S-allele-specific discrimination. Copyright (C) 1998 Federation of European Biochemical Societies.
KW - Non-synonymous nucleotide substitution
KW - Positive selection
KW - Rosacea
KW - S-RNase
KW - Self-incompatibility
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U2 - 10.1016/S0014-5793(98)01470-7
DO - 10.1016/S0014-5793(98)01470-7
M3 - Article
C2 - 9872398
AN - SCOPUS:0032437156
VL - 440
SP - 337
EP - 342
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 3
ER -