Abstract
Yeast two-hybrid screening of mouse cDNA libraries was performed to identify proteins interacting with selenocysteine lyase (SCL), which decomposes L-selenocysteine. Several proteins related to spermatogenesis, protein synthesis, and cell viability/apoptosis were identified as potential interactors. Major urinary proteins 1 and 2 interacted with SCL and inhibited its activity. Coimmunoprecipitation revealed interactions between SCL and each of two selenophosphate synthetase isozymes.
| Original language | English |
|---|---|
| Pages (from-to) | 1230-1232 |
| Number of pages | 3 |
| Journal | Bioscience, Biotechnology and Biochemistry |
| Volume | 73 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2009 |
| Externally published | Yes |
Keywords
- Major urinary protein
- Protein-protein interaction
- Selenocysteine lyase
- Selenophosphate synthetase
- Yeast two-hybrid system
ASJC Scopus subject areas
- Biotechnology
- Analytical Chemistry
- Biochemistry
- Applied Microbiology and Biotechnology
- Molecular Biology
- Organic Chemistry