Identification of proteins interacting with selenocysteine lyase

Ryuta Tobe, Hisaaki Mihara, Tatsuo Kurihara, Nobuyoshi Esaki

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)


Yeast two-hybrid screening of mouse cDNA libraries was performed to identify proteins interacting with selenocysteine lyase (SCL), which decomposes L-selenocysteine. Several proteins related to spermatogenesis, protein synthesis, and cell viability/apoptosis were identified as potential interactors. Major urinary proteins 1 and 2 interacted with SCL and inhibited its activity. Coimmunoprecipitation revealed interactions between SCL and each of two selenophosphate synthetase isozymes.

Original languageEnglish
Pages (from-to)1230-1232
Number of pages3
JournalBioscience, Biotechnology and Biochemistry
Issue number5
Publication statusPublished - 2009
Externally publishedYes


  • Major urinary protein
  • Protein-protein interaction
  • Selenocysteine lyase
  • Selenophosphate synthetase
  • Yeast two-hybrid system

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry


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