Identification of protein tyrosine phosphatases expressed in murine male germ cells

Yoshiyuki Kaneko, Seiichiro Takano, Kazuhiro Okumura, Jun Takenawa, Hiroaki Higashituji, Manabu Fukumoto, Hiroki Nakayama, Jun Fujita

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

We cloned five cDNAs encoding putative protein tyrosine phosphatases (PTPs; protein-tyrosine-phosphatase phosphohydrolase, EC 3.1.3.48) from the murine testis by using degenerate primers and the polymerase chain reaction cloning technique. Two of them were identical to the mouse cytoplasmic PTP and PTP-1B. The remaining three were likely to represent the murine counterparts of human FTP δ, human FTP ε(lunate), and rat striatum-enriched PTP. The cells expressing these genes were determined either by in situ hybridization histochemistry or by Northern blot hybridization. Moreover, Northern blot hybridization revealed that the transcripts of FTP-1B, mouse cytoplasmic FTP and the murine homologue of human PTP δ were quantitatively and/or structurally regulated during germ cell development, suggesting their roles in spermatogenesis.

Original languageEnglish
Pages (from-to)625-631
Number of pages7
JournalBiochemical and biophysical research communications
Volume197
Issue number2
DOIs
Publication statusPublished - 1993 Dec 15

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Kaneko, Y., Takano, S., Okumura, K., Takenawa, J., Higashituji, H., Fukumoto, M., Nakayama, H., & Fujita, J. (1993). Identification of protein tyrosine phosphatases expressed in murine male germ cells. Biochemical and biophysical research communications, 197(2), 625-631. https://doi.org/10.1006/bbrc.1993.2525