Identification of potential serum markers for endometrial cancer using protein expression profiling

Masashi Takano, Yoshihiro Kikuchi, Takayoshi Asakawa, Tomoko Goto, Tsunekazu Kita, Kazuya Kudoh, Junzo Kigawa, Noriaki Sakuragi, Masaru Sakamoto, Toru Sugiyama, Nobuo Yaegashi, Hiroshi Tsuda, Hiroshi Seto, Mieko Shiwa

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

Objectives: Screening method of endometrial cancer (EC) has not been established yet. Our study was to explore serum biomarkers of EC patients using surface-enhanced laser desorption and ionization-time-of-flight mass spectrometry (SELDI-TOF MS). Methods: Serum samples from 65 EC patients and 40 controls were analyzed by SELDI-TOF MS (training set). Single- and multi-variant analyses were performed to compare protein profiles in serum of EC patients and healthy controls. Subsequently, blind test set including 40 EC patients and 40 controls were analyzed for validation. Results: A panel of four biomarker candidates were selected in training set analysis. These markers could also distinguish stage I patients from controls. Among them, two biomarkers were purified and identified as apolipoprotein A1 and a modified form of apolipoprotein C1. Screening for blind test set using dual-biomarker analysis yielded a sensitivity of 82% and a specificity of 86%. Conclusions: Involvement of apolipoproteins with EC is first suggested in this study. In addition to possibility of screening method for EC, findings of these new biomarkers might be related with carcinogenesis or predisposition to EC.

Original languageEnglish
Pages (from-to)475-481
Number of pages7
JournalJournal of Cancer Research and Clinical Oncology
Volume136
Issue number3
DOIs
Publication statusPublished - 2010 Mar

Keywords

  • Apolipoprotein
  • Endometrial cancer
  • Proteomics
  • Screening
  • Serum biomarker

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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