Identification of potential biomarkers of radiation exposure in blood cells by capillary electrophoresis time-of-flight mass spectrometry

Lue Sun, Yohei Inaba, Norie Kanzaki, Mahesh Bekal, Koichi Chida, Takashi Moritake

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Biodosimetry is a useful method for estimating personal exposure doses to ionizing radiation. Studies have identified metabolites in non-cellular biofluids that can be used as markers in biodosimetry. Levels of metabolites in blood cells may reflect health status or environmental stresses differentially. Here, we report changes in the levels of murine blood cell metabolites following exposure to X-rays in vivo. Levels of blood cell metabolites were measured by capillary electrophoresis time-of-flight mass spectrometry. The levels of 100 metabolites were altered substantially following exposure. We identified 2-aminobutyric acid, 2-deoxycytidine, and choline as potentially useful markers of radiation exposure and established a potential prediction panel of the exposure dose using stepwise regression. Levels of blood cell metabolites may be useful biomarkers in estimating exposure doses during unexpected radiation incidents.

Original languageEnglish
Article number812
JournalInternational journal of molecular sciences
Volume21
Issue number3
DOIs
Publication statusPublished - 2020 Feb

Keywords

  • Biodosimetry
  • Blood cell
  • Disaster medicine
  • Metabolome
  • Radiation

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Fingerprint Dive into the research topics of 'Identification of potential biomarkers of radiation exposure in blood cells by capillary electrophoresis time-of-flight mass spectrometry'. Together they form a unique fingerprint.

Cite this