Identification of polymorphisms in the promoter region of the human NRF2 gene

Tae Yamamoto, Keigyou Yoh, Akira Kobayashi, Yukio Ishii, Shigeo Kure, Akio Koyama, Tohru Sakamoto, Kiyohisa Sekizawa, Hozumi Motohashi, Masayuki Yamamoto

Research output: Contribution to journalArticlepeer-review

106 Citations (Scopus)

Abstract

Transcription factor Nrf2 regulates the basal and inducible expression of detoxifying and antioxidant genes. Recent studies using nrf2-null mice suggest that Nrf2 dysfunction might be involved in the pathogenesis of human diseases. To gain insight into the relationship between impairment in the NRF2 gene and human diseases, we attempted to identify polymorphisms in the human NRF2 gene. We determined the structure of the NRF2 gene and found three single nucleotide polymorphisms and one triplet repeat polymorphism in its regulatory region. These results provide a molecular basis for the genetic analysis of the NRF2 gene. The frequency of each polymorphism was examined in two groups of patients with systemic lupus erythematosus and chronic obstructive pulmonary disease. This study did not reveal a close connection between the risk of these diseases and the polymorphisms. However, available lines of evidence suggest the importance of examining the link between NRF2 polymorphisms and other oxidative stress-related diseases.

Original languageEnglish
Pages (from-to)72-79
Number of pages8
JournalBiochemical and biophysical research communications
Volume321
Issue number1
DOIs
Publication statusPublished - 2004 Aug 13

Keywords

  • 5′-RACE
  • Chronic obstructive pulmonary diseases
  • Human NRF2 gene
  • Initiator site
  • Japanese
  • Polymorphism
  • Promoter
  • RNase protection
  • SNP
  • Systemic lupus erythematosus
  • Transcription

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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