Identification of pin1-binding phosphorylated proteins in the mouse brain

Yota Tatara, Takahiro Terakawa, Takafumi Uchida

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

To determine the role of Pin1 in the neurotransmission pathway, Pin1-binding proteins in mouse brain extract were identified. The Pin1-binding proteins were extracted from mouse brain homogenate, and the trypsin-digested peptides were analyzed by nano-liquid chromatography tandem mass spectrometry (LC-MS/ MS). Proteins that involve the neurotransmission pathway, such as synapsin I, synapsin II, and calcium/ calmodulin-dependent protein kinase type II (CaMKII), were identified in a Mascot search. Pull-down and immunoprecipitation assay indicated that Pin1 binds CaMKII in a phosphorylation-specific manner. It was assumed that Pin1 participates in the neurotransmission pathway involving the phosphorylation signal by CaMKII.

Original languageEnglish
Pages (from-to)2480-2483
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume74
Issue number12
DOIs
Publication statusPublished - 2010 Dec 1

Keywords

  • Calcium/calmodulindependent protein kinase type II
  • Neurotransmission
  • Peptidyl prolyl cis/trans isomerase
  • Phosphorylation
  • Pin1

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Analytical Chemistry
  • Organic Chemistry

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