Identification of ophiobolin F synthase by a genome mining approach: A sesterterpene synthase from aspergillus clavatus

Ryota Chiba, Atsushi Minami, Katsuya Gomi, Hideaki Oikawa

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

During a screening of putative diterpene synthase genes found in public databases using the Aspergillus oryzae expression system, it was found that a single transformant with the ACLA-76850 gene from A. clavatus produced a sesterterpene alcohol, ophiobolin F, and three minor sesterterpene hydrocarbons. The sesterterpene synthase has two catalytically independent domains (prenyltransferase/terpene cyclase) which are homologous to those of diterpene synthase, fusicoccadiene synthase. Coevolution of both domains and reaction mechanisms of these terpene synthases are discussed.

Original languageEnglish
Pages (from-to)594-597
Number of pages4
JournalOrganic letters
Volume15
Issue number3
DOIs
Publication statusPublished - 2013 Feb 1

ASJC Scopus subject areas

  • Biochemistry
  • Physical and Theoretical Chemistry
  • Organic Chemistry

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