Abstract
During a screening of putative diterpene synthase genes found in public databases using the Aspergillus oryzae expression system, it was found that a single transformant with the ACLA-76850 gene from A. clavatus produced a sesterterpene alcohol, ophiobolin F, and three minor sesterterpene hydrocarbons. The sesterterpene synthase has two catalytically independent domains (prenyltransferase/terpene cyclase) which are homologous to those of diterpene synthase, fusicoccadiene synthase. Coevolution of both domains and reaction mechanisms of these terpene synthases are discussed.
| Original language | English |
|---|---|
| Pages (from-to) | 594-597 |
| Number of pages | 4 |
| Journal | Organic letters |
| Volume | 15 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2013 Feb 1 |
ASJC Scopus subject areas
- Biochemistry
- Physical and Theoretical Chemistry
- Organic Chemistry
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Chiba, R., Minami, A., Gomi, K., & Oikawa, H. (2013). Identification of ophiobolin F synthase by a genome mining approach: A sesterterpene synthase from aspergillus clavatus. Organic letters, 15(3), 594-597. https://doi.org/10.1021/ol303408a