Identification of ophiobolin F synthase by a genome mining approach: A sesterterpene synthase from aspergillus clavatus

Ryota Chiba; Atsushi Minami; Katsuya Gomi; Hideaki Oikawa

doi:10.1021/ol303408a

Identification of ophiobolin F synthase by a genome mining approach: A sesterterpene synthase from aspergillus clavatus

Ryota Chiba, Atsushi Minami, Katsuya Gomi, Hideaki Oikawa

Research output: Contribution to journal › Article › peer-review

104 Citations (Scopus)

Abstract

During a screening of putative diterpene synthase genes found in public databases using the Aspergillus oryzae expression system, it was found that a single transformant with the ACLA-76850 gene from A. clavatus produced a sesterterpene alcohol, ophiobolin F, and three minor sesterterpene hydrocarbons. The sesterterpene synthase has two catalytically independent domains (prenyltransferase/terpene cyclase) which are homologous to those of diterpene synthase, fusicoccadiene synthase. Coevolution of both domains and reaction mechanisms of these terpene synthases are discussed.

Original language	English
Pages (from-to)	594-597
Number of pages	4
Journal	Organic letters
Volume	15
Issue number	3
DOIs	https://doi.org/10.1021/ol303408a
Publication status	Published - 2013 Feb 1

ASJC Scopus subject areas

Biochemistry
Physical and Theoretical Chemistry
Organic Chemistry

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AU - Oikawa, Hideaki

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Identification of ophiobolin F synthase by a genome mining approach: A sesterterpene synthase from aspergillus clavatus

Abstract

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