Identification of ophiobolin F synthase by a genome mining approach: A sesterterpene synthase from aspergillus clavatus

Ryota Chiba, Atsushi Minami, Katsuya Gomi, Hideaki Oikawa

    Research output: Contribution to journalArticlepeer-review

    104 Citations (Scopus)

    Abstract

    During a screening of putative diterpene synthase genes found in public databases using the Aspergillus oryzae expression system, it was found that a single transformant with the ACLA-76850 gene from A. clavatus produced a sesterterpene alcohol, ophiobolin F, and three minor sesterterpene hydrocarbons. The sesterterpene synthase has two catalytically independent domains (prenyltransferase/terpene cyclase) which are homologous to those of diterpene synthase, fusicoccadiene synthase. Coevolution of both domains and reaction mechanisms of these terpene synthases are discussed.

    Original languageEnglish
    Pages (from-to)594-597
    Number of pages4
    JournalOrganic letters
    Volume15
    Issue number3
    DOIs
    Publication statusPublished - 2013 Feb 1

    ASJC Scopus subject areas

    • Biochemistry
    • Physical and Theoretical Chemistry
    • Organic Chemistry

    Fingerprint

    Dive into the research topics of 'Identification of ophiobolin F synthase by a genome mining approach: A sesterterpene synthase from aspergillus clavatus'. Together they form a unique fingerprint.

    Cite this