Identification of novel mast cell genes by serial analysis of gene expression in cord blood-derived mast cells

Atsuo Kuramasu; Yasuhiko Kubota; Kenji Matsumoto; Toshiharu Nakajima; Xu Ming Sun; Takehiko Watanabe; Hirohisa Saito; Hiroshi Ohtsu

doi:10.1016/S0014-5793(01)02477-2

Identification of novel mast cell genes by serial analysis of gene expression in cord blood-derived mast cells

Atsuo Kuramasu, Yasuhiko Kubota, Kenji Matsumoto, Toshiharu Nakajima, Xu Ming Sun, Takehiko Watanabe, Hirohisa Saito, Hiroshi Ohtsu

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

The gene expression profile of human cord blood-derived mast cells (MCs) was investigated using serial analysis of gene expression (SAGE). A total of 22914 tags, representing 9181 unique transcripts, were sequenced. By selecting tags that were detected more frequently in MCs than in other tissues, genes characteristic of MCs were enriched. Reverse transcription-PCR and the high-density oligonucleotide array hybridization confirmed the validity of our SAGE result. About 70% of the selected genes were previously uncharacterized. Northern blot analysis showed the MC-specific expression of selected genes. This inventory will be useful to identify novel genes with important functions in MCs.

Original language	English
Pages (from-to)	37-41
Number of pages	5
Journal	FEBS Letters
Volume	498
Issue number	1
DOIs	https://doi.org/10.1016/S0014-5793(01)02477-2
Publication status	Published - 2001 Jun 1
Externally published	Yes

Keywords

Human
Mast cell
Serial analysis of gene expression
Transcript

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/S0014-5793(01)02477-2

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abstract = "The gene expression profile of human cord blood-derived mast cells (MCs) was investigated using serial analysis of gene expression (SAGE). A total of 22914 tags, representing 9181 unique transcripts, were sequenced. By selecting tags that were detected more frequently in MCs than in other tissues, genes characteristic of MCs were enriched. Reverse transcription-PCR and the high-density oligonucleotide array hybridization confirmed the validity of our SAGE result. About 70% of the selected genes were previously uncharacterized. Northern blot analysis showed the MC-specific expression of selected genes. This inventory will be useful to identify novel genes with important functions in MCs.",

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AU - Kuramasu, Atsuo

AU - Kubota, Yasuhiko

AU - Matsumoto, Kenji

AU - Nakajima, Toshiharu

AU - Sun, Xu Ming

AU - Watanabe, Takehiko

AU - Saito, Hirohisa

AU - Ohtsu, Hiroshi

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Identification of novel mast cell genes by serial analysis of gene expression in cord blood-derived mast cells

Abstract

Keywords

ASJC Scopus subject areas

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