TY - JOUR
T1 - Identification of multiple actin-binding sites in cofilin-phosphatase Slingshot-1L
AU - Yamamoto, Masahiro
AU - Nagata-Ohashi, Kyoko
AU - Ohta, Yusaku
AU - Ohashi, Kazumasa
AU - Mizuno, Kensaku
N1 - Funding Information:
We thank S. Kurita for providing SSH1L purified from baculovirus expression system, and R. Niwa and T. Uemura for helpful comments. This work was supported by a Grant-in-Aid for Creative Scientific Research from the Japan Society for the Promotion of Science.
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2006/3/20
Y1 - 2006/3/20
N2 - Slingshot-1L (SSH1L) is a phosphatase that specifically dephosphorylates and activates cofilin, an actin-severing and -depolymerizing protein. SSH1L binds to and is activated by F-actin in vitro, and co-localizes with F-actin in cultured cells. We examined the F-actin-binding activity, F-actin-mediated phosphatase activation, and subcellular distribution of various mutants of SSH1L. We identified three sites involved in F-actin binding of SSH1L: Trp-458 close to the C-terminus of the phosphatase domain, an LHK motif in the N-terminal region, and an LKR motif in the C-terminal region. These sites play unique roles in the control of subcellular localization and F-actin-mediated activation of SSH1L.
AB - Slingshot-1L (SSH1L) is a phosphatase that specifically dephosphorylates and activates cofilin, an actin-severing and -depolymerizing protein. SSH1L binds to and is activated by F-actin in vitro, and co-localizes with F-actin in cultured cells. We examined the F-actin-binding activity, F-actin-mediated phosphatase activation, and subcellular distribution of various mutants of SSH1L. We identified three sites involved in F-actin binding of SSH1L: Trp-458 close to the C-terminus of the phosphatase domain, an LHK motif in the N-terminal region, and an LKR motif in the C-terminal region. These sites play unique roles in the control of subcellular localization and F-actin-mediated activation of SSH1L.
KW - Actin-binding
KW - Cofilin
KW - Phosphatase
KW - Slingshot
UR - http://www.scopus.com/inward/record.url?scp=33644906129&partnerID=8YFLogxK
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U2 - 10.1016/j.febslet.2006.02.034
DO - 10.1016/j.febslet.2006.02.034
M3 - Article
C2 - 16513117
AN - SCOPUS:33644906129
VL - 580
SP - 1789
EP - 1794
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 7
ER -