Identification of mRNAs differentially expressed between embryogenic and non-embryogenic cultivars of eggplant during somatic embryogenesis

John Cliff Afele; Yutaka Tabei; Tomohiro Yamada; Takahiro Momiyama; Fumio Takaiwa; Toshiaki Kayano; Shigeo Nishimura; Takeshi Nishio

Identification of mRNAs differentially expressed between embryogenic and non-embryogenic cultivars of eggplant during somatic embryogenesis

John Cliff Afele, Yutaka Tabei, Tomohiro Yamada, Takahiro Momiyama, Fumio Takaiwa, Toshiaki Kayano, Shigeo Nishimura, Takeshi Nishio

AGR - Life Sciences

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

The molecular basis of differential cultivar response to the induction of somatic embryogenesis was investigated by using the digoxigenin (DIG)-differential display with 2 eggplant (Solanum melongena L.) cultivars, Wase Shinkuro (WS) and Kumamoto Naga (KN). WS produced embryogenic callus (EC) which was capable of differentiating into mature somatic embryos while KN cultures treated similarly failed to differentiate somatic embryos. DIG-differential display of 0, 1, 2, 4, and 10-day old cultures of both cultivars revealed several PCR products derived from WS, but not from KN, at a crucial stage during embryogenesis. These results indicated that the cultivar difference in EC induction may be attributed, in part, to differences in mRNA expression between the cultivars during the culture process.

Original language	English
Pages (from-to)	175-179
Number of pages	5
Journal	Japan Agricultural Research Quarterly
Volume	30
Issue number	3
Publication status	Published - 1996 Jul 1

Keywords

DIG-differential display
PCR
Solanum melongena L

ASJC Scopus subject areas

Biotechnology
Ecology
Animal Science and Zoology
Agronomy and Crop Science

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Identification of mRNAs differentially expressed between embryogenic and non-embryogenic cultivars of eggplant during somatic embryogenesis. / Afele, John Cliff; Tabei, Yutaka; Yamada, Tomohiro; Momiyama, Takahiro; Takaiwa, Fumio; Kayano, Toshiaki; Nishimura, Shigeo; Nishio, Takeshi.

In: Japan Agricultural Research Quarterly, Vol. 30, No. 3, 01.07.1996, p. 175-179.

Research output: Contribution to journal › Article › peer-review

Afele, John Cliff ; Tabei, Yutaka ; Yamada, Tomohiro ; Momiyama, Takahiro ; Takaiwa, Fumio ; Kayano, Toshiaki ; Nishimura, Shigeo ; Nishio, Takeshi. / Identification of mRNAs differentially expressed between embryogenic and non-embryogenic cultivars of eggplant during somatic embryogenesis. In: Japan Agricultural Research Quarterly. 1996 ; Vol. 30, No. 3. pp. 175-179.

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abstract = "The molecular basis of differential cultivar response to the induction of somatic embryogenesis was investigated by using the digoxigenin (DIG)-differential display with 2 eggplant (Solanum melongena L.) cultivars, Wase Shinkuro (WS) and Kumamoto Naga (KN). WS produced embryogenic callus (EC) which was capable of differentiating into mature somatic embryos while KN cultures treated similarly failed to differentiate somatic embryos. DIG-differential display of 0, 1, 2, 4, and 10-day old cultures of both cultivars revealed several PCR products derived from WS, but not from KN, at a crucial stage during embryogenesis. These results indicated that the cultivar difference in EC induction may be attributed, in part, to differences in mRNA expression between the cultivars during the culture process.",

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AU - Afele, John Cliff

AU - Tabei, Yutaka

AU - Yamada, Tomohiro

AU - Momiyama, Takahiro

AU - Takaiwa, Fumio

AU - Kayano, Toshiaki

AU - Nishimura, Shigeo

AU - Nishio, Takeshi

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AB - The molecular basis of differential cultivar response to the induction of somatic embryogenesis was investigated by using the digoxigenin (DIG)-differential display with 2 eggplant (Solanum melongena L.) cultivars, Wase Shinkuro (WS) and Kumamoto Naga (KN). WS produced embryogenic callus (EC) which was capable of differentiating into mature somatic embryos while KN cultures treated similarly failed to differentiate somatic embryos. DIG-differential display of 0, 1, 2, 4, and 10-day old cultures of both cultivars revealed several PCR products derived from WS, but not from KN, at a crucial stage during embryogenesis. These results indicated that the cultivar difference in EC induction may be attributed, in part, to differences in mRNA expression between the cultivars during the culture process.

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