Identification of mRNAs differentially expressed between embryogenic and non-embryogenic cultivars of eggplant during somatic embryogenesis

John Cliff Afele, Yutaka Tabei, Tomohiro Yamada, Takahiro Momiyama, Fumio Takaiwa, Toshiaki Kayano, Shigeo Nishimura, Takeshi Nishio

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

The molecular basis of differential cultivar response to the induction of somatic embryogenesis was investigated by using the digoxigenin (DIG)-differential display with 2 eggplant (Solanum melongena L.) cultivars, Wase Shinkuro (WS) and Kumamoto Naga (KN). WS produced embryogenic callus (EC) which was capable of differentiating into mature somatic embryos while KN cultures treated similarly failed to differentiate somatic embryos. DIG-differential display of 0, 1, 2, 4, and 10-day old cultures of both cultivars revealed several PCR products derived from WS, but not from KN, at a crucial stage during embryogenesis. These results indicated that the cultivar difference in EC induction may be attributed, in part, to differences in mRNA expression between the cultivars during the culture process.

Original languageEnglish
Pages (from-to)175-179
Number of pages5
JournalJapan Agricultural Research Quarterly
Volume30
Issue number3
Publication statusPublished - 1996 Jul 1

Keywords

  • DIG-differential display
  • PCR
  • Solanum melongena L

ASJC Scopus subject areas

  • Biotechnology
  • Ecology
  • Animal Science and Zoology
  • Agronomy and Crop Science

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