Identification of mRNAs differentially expressed between embryogenic and non-embryogenic cultivars of eggplant during somatic embryogenesis

John Cliff Afele, Yutaka Tabei, Tomohiro Yamada, Takahiro Momiyama, Fumio Takaiwa, Toshiaki Kayano, Shigeo Nishimura, Takeshi Nishio

    Research output: Contribution to journalArticlepeer-review

    5 Citations (Scopus)

    Abstract

    The molecular basis of differential cultivar response to the induction of somatic embryogenesis was investigated by using the digoxigenin (DIG)-differential display with 2 eggplant (Solanum melongena L.) cultivars, Wase Shinkuro (WS) and Kumamoto Naga (KN). WS produced embryogenic callus (EC) which was capable of differentiating into mature somatic embryos while KN cultures treated similarly failed to differentiate somatic embryos. DIG-differential display of 0, 1, 2, 4, and 10-day old cultures of both cultivars revealed several PCR products derived from WS, but not from KN, at a crucial stage during embryogenesis. These results indicated that the cultivar difference in EC induction may be attributed, in part, to differences in mRNA expression between the cultivars during the culture process.

    Original languageEnglish
    Pages (from-to)175-179
    Number of pages5
    JournalJapan Agricultural Research Quarterly
    Volume30
    Issue number3
    Publication statusPublished - 1996 Jul 1

    Keywords

    • DIG-differential display
    • PCR
    • Solanum melongena L

    ASJC Scopus subject areas

    • Biotechnology
    • Ecology
    • Animal Science and Zoology
    • Agronomy and Crop Science

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