Identification of mouse orthologue of endogenous secretory receptor for advanced glycation end-products: Structure, function and expression

Ai Harashima, Yasuhiko Yamamoto, Chunmei Cheng, Koichi Tsuneyama, Khin Mar Myint, Akihiko Takeuchi, Kazunobu Yoshimura, Hui Li, Takuo Watanabe, Shin Takasawa, Hiroshi Okamoto, Hideto Yonekura, Hiroshi Yamamoto

Research output: Contribution to journalArticlepeer-review

44 Citations (Scopus)

Abstract

The cell-surface RAGE [receptor for AGE (advanced glycation end-products)] is associated with the development of diabetic vascular complications, neurodegenerative disorders and inflammation. Recently, we isolated a human RAGE splice variant, which can work as a decoy receptor for RAGE ligands, and named it esRAGE (endogenous secretory RAGE). In the present study, we have isolated the murine equivalent of esRAGE from brain polysomal poly(A)+ (polyadenylated) RNA by RT (reverse transcription)-PCR cloning. The mRNA was generated by alternative splicing, and it encoded a 334-amino-acid protein with a signal sequence, but lacking the transmembrane domain. A transfection experiment revealed that the mRNA was actually translated as deduced to yield the secretory protein working as a decoy in AGE-induced NF-κB (nuclear factor κB) activation. RT-PCR and immunoblotting detected esRAGE mRNA and protein in the brain, lung, kidney and small intestine of wild-type mice, but not of RAGE-null mice. The esRAGE expression was increased in the kidney of diabetic wild-type mice. The present study has thus provided an animal orthologue of esRAGE for clarification of its roles in health and disease.

Original languageEnglish
Pages (from-to)109-115
Number of pages7
JournalBiochemical Journal
Volume396
Issue number1
DOIs
Publication statusPublished - 2006 May 15

Keywords

  • Advanced glycation end-product
  • Endogenous secretory receptor for advanced glycation end-products (esRAGE)
  • Immunohistochemistry
  • Reverse transcription-PCR cloning

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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