Identification of mother trees based on microsatellite analysis of maternal tissues from seeds, fruits, and seedlings

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Abstract

The availability of hypervariable microsatellite markers has greatly extended the possibility of parentage analysis in natural plant populations. However, maternity and paternity analysis based on DNA from biparental tissues is often difficult when both parents are unknown and many candidates are available. In this paper, I present a new approach for the identification of the mother trees based on microsatellite analysis of maternal tissues from seeds, fruits, and seedlings. By comparing the maternal genotype with the genotypes of the adult trees, it is possible to unambiguously identify mother trees from the candidates in a population. Because DNA extraction from particular maternal tissues such as woody pericarps is technically complicated, different DNA extraction methods and PCR amplification procedures should be tested on each tissue before performing routine analyses. By choosing the best laboratory procedures based on what tissue is to be analyzed, studying seed dispersal as well as the relative fertility of individual trees in a forest is possible.

Original languageEnglish
Pages (from-to)177-183
Number of pages7
JournalNihon Ringakkai Shi/Journal of the Japanese Forestry Society
Volume86
Issue number2
Publication statusPublished - 2004 Dec 1

Keywords

  • Fagus crenata
  • Parentage analysis
  • Pericarp
  • Reproductive success
  • Seed dispersal

ASJC Scopus subject areas

  • Forestry

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