TY - JOUR
T1 - Identification of molting fluid carboxypeptidase A (MF-CPA) in Bombyx mori
AU - Ote, Manabu
AU - Mita, Kazuei
AU - Kawasaki, Hideki
AU - Daimon, Takaaki
AU - Kobayashi, Masahiko
AU - Shimada, Toru
N1 - Funding Information:
We thank Naoko Omuro for technical assistance. This work was supported by grants from BRAIN (to M.K. and T.S.), MEXT (Nos. 16011209 and 00150012) (to T.S.), and MAFF-NIAS (Insect Technology) (to T.S.), Japan.
PY - 2005/7
Y1 - 2005/7
N2 - Using microarray analyses, we identified carboxypeptidase A (MF-CPA), which was induced during pupal ecdysis in the wing discs of Bombyx mori. Here, we report the functional characterization of MF-CPA. MF-CPA has amino acid sequence similarities with the proteins in the carboxypeptidase A/B subfamily, from human to nematode. The MF-CPA gene is expressed during the molting periods in the epithelial tissues. MF-CPA is detected in the molting fluid, which fills the space between the old and new cuticle during molting. By Western blot analysis, we show that MF-CPA is secreted as a zymogen and processed in the molting fluid. Recombinant MF-CPA expressed in the insect cells has carboxypeptidase A activity. We propose that MF-CPA degrades the proteins from the old cuticle during the molting periods and contributes to recycling of the amino acids.
AB - Using microarray analyses, we identified carboxypeptidase A (MF-CPA), which was induced during pupal ecdysis in the wing discs of Bombyx mori. Here, we report the functional characterization of MF-CPA. MF-CPA has amino acid sequence similarities with the proteins in the carboxypeptidase A/B subfamily, from human to nematode. The MF-CPA gene is expressed during the molting periods in the epithelial tissues. MF-CPA is detected in the molting fluid, which fills the space between the old and new cuticle during molting. By Western blot analysis, we show that MF-CPA is secreted as a zymogen and processed in the molting fluid. Recombinant MF-CPA expressed in the insect cells has carboxypeptidase A activity. We propose that MF-CPA degrades the proteins from the old cuticle during the molting periods and contributes to recycling of the amino acids.
KW - Bombyx mori
KW - Carboxypeptidase
KW - MF-CPA
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U2 - 10.1016/j.cbpc.2005.04.005
DO - 10.1016/j.cbpc.2005.04.005
M3 - Article
C2 - 15936966
AN - SCOPUS:20544455530
VL - 141
SP - 314
EP - 322
JO - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
JF - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
SN - 1096-4959
IS - 3
ER -