Identification of Metal-Binding Peptides and Their Conjugation onto Nanoparticles of Superparamagnetic Iron Oxides and Liposomes

Tomonobu Kodama, Tomonobu Kodama, Akifumi Yoshihara, Isha Goel, Masaki Sekino, Masaki Sekino, Akihiro Kuwahata, Atsushi Yoshimori, Yuichi Murayama, Kazuhiko Ishihara, Kristina N. Ekdahl, Kristina N. Ekdahl, Bo Nilsson, Yuji Teramura, Yuji Teramura

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


Metallic materials are used for clinical medical devices such as vascular stents and coils to treat both ischemic and hemorrhagic vascular diseases. An antiplatelet drug is required to avoid thromboembolic complication until metallic surface is covered with a neo-endothelial cell layer. It is important to identify endothelial cell coverage on the metallic surface. However, it is difficult since there are no selective ligands. Here, we used the phage display method to identify peptide ligands that had high affinity for the metallic surface of Ni-Ti stents, Pt-W coils, and Co-Cr stents. The binding assay using fluorescence labeling revealed that several synthetic peptides could bind onto those surfaces. We also chose some oligopeptides for the conjugation onto superparamagnetic iron oxide (SPIO) nanoparticles and liposome-encapsulating SPIO nanoparticles and studied their ability to bind to the stent and coils. By SEM and fluorophotometry, we found that those modified SPIOs and liposomes were selectively bound onto those surfaces. In addition, both treated stents and coils could be detected by magnetic resonance imaging due to the magnetic artifact through the SPIOs and liposomes that were immobilized onto the surface. Thus, we identified metal-binding peptides which may enable to stop antiplatelet therapy after vascular stenting or coiling.

Original languageEnglish
Pages (from-to)24623-24634
Number of pages12
JournalACS Applied Materials and Interfaces
Issue number22
Publication statusPublished - 2020 Jun 3
Externally publishedYes


  • SPIO
  • coil
  • liposome
  • metal-binding peptide
  • phage display
  • stent

ASJC Scopus subject areas

  • Materials Science(all)


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