Identification of loci associated with embryo yield in microspore culture of Brassica rapa by segregation distortion analysis

Hiroyasu Kitashiba, Kumiko Taguchi, Ikuyo Kaneko, Kiyofumi Inaba, Shuji Yokoi, Yoshihito Takahata, Takeshi Nishio

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Key message: We identified three physical positions associated with embryo yield in microspore culture ofBrassica rapaby segregation distortion analysis. We also confirmed their genetic effects on the embryo yield. Abstract: Isolated microspore culture is well utilized for the production of haploid or doubled-haploid plants in Brassica crops. Brassica rapa cv. ‘Ho Mei’ is one of the most excellent cultivars in embryo yield of microspore culture. To identify the loci associated with microspore embryogenesis, segregation analysis of 154 DNA markers anchored to B. rapa chromosomes (A01–A10) was performed using a population of microspore-derived embryos obtained from an F1 hybrid between ‘CR-Seiga’, a low yield cultivar in microspore-derived embryos, and ‘Ho Mei’. Three regions showing significant segregation distortion with increasing ‘Ho Mei’ alleles were detected on A05, A08 and A09, although these regions showed the expected Mendelian segregation ratio in an F2 population. The additive effect of alleles in these regions on embryo yield was confirmed in a BC3F1 population. One region on A08 containing Br071-5c had a higher effect than the other regions. Polymorphism of nucleotide sequences around the Br071-5c locus was investigated to find the gene possibly responsible for efficient embryogenesis from microspores.

Original languageEnglish
Pages (from-to)2197-2204
Number of pages8
JournalPlant Cell Reports
Volume35
Issue number10
DOIs
Publication statusPublished - 2016 Oct 1

Keywords

  • Brassica rapa
  • Microspore culture
  • Microspore-derived embryo
  • Segregation distortion

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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