Identification of L-glutamine: 2-deoxy-scyllo-inosose-aminotransferase required for the biosynthesis of butirosin in Bacillus circulans

Hideyuki Tamegai, Eriko Nango, Mieko Kuwahara, Hideki Yamamoto, Yasumasa Ota, Hisako Kuriki, Tadashi Eguchi, Katsumi Kakinuma

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Using inverse PCR, two new genes (btrN and btrS) were identified upstream of the purative glycosyltransferase gene btrM in the butirosin-biosynthetic btr gene cluster of Bacillus circulans. The upstream gene btrS showed significant homology with stsC of Streptomyces griseus, which encodes L-glutamine : scyllo-inosose aminotransferase in the biosynthesis of streptomycin. The function of BtrS was further confirmed by heterologous expression in Escherichia coii and chemical identification of the conversion of 2-deoxy-scyllo-inosose into 2-deoxy-scyllo-inosamine. The identification of BtrS as L-glutamine: 2-deoxy-scyllo-inosose aminotransferase is the first report of the aminotransferase gene responsible for 2-deoxystreptamine biosynthesis.

Original languageEnglish
Pages (from-to)707-714
Number of pages8
JournalJournal of Antibiotics
Volume55
Issue number8
DOIs
Publication statusPublished - 2002 Aug
Externally publishedYes

ASJC Scopus subject areas

  • Pharmacology
  • Drug Discovery

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