Identification of KLF9 and BCL3 as transcription factors that enhance reprogramming of primordial germ cells

Kei Otsuka, Asuka Takehara, Natsuko Chiba, Yasuhisa Matsui

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


Primordial germ cells (PGCs) are precursors of eggs and sperm. Although PGCs are unipotent cells in vivo, they are reprogrammed into pluripotent stem cells (PSCs), also known as embryonic germ cells (EGCs), in the presence of leukemia inhibitory factor and basic fibroblast growth factor (bFGF) in vitro. However, the molecular mechanisms responsible for their reprogramming are not fully understood. Here we show identification of transcription factors that mediate PGC reprogramming. We selected genes encoding transcription factors or epigenetic regulatory factors whose expression was significantly different between PGCs and PSCs with in silico analysis and RT-qPCR. Among the candidate genes, overexpression (OE) of Bcl3 or Klf9 significantly enhanced PGC reprogramming. Notably, EGC formation was stimulated by Klf9-OE even without bFGF. G-protein-coupled receptor signaling- related pathways, which are involved in PGC reprogramming, were enriched among genes down-regulated by Klf9-OE, and forskolin which activate adenylate cyclase, rescued repressed EGC formation by knock-down of Klf9, suggesting a molecular linkage between KLF9 and such signaling.

Original languageEnglish
Article numbere0205004
JournalPloS one
Issue number10
Publication statusPublished - 2018 Oct

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General


Dive into the research topics of 'Identification of KLF9 and BCL3 as transcription factors that enhance reprogramming of primordial germ cells'. Together they form a unique fingerprint.

Cite this