Identification of insertion sequence from a γ-hexachlorocyclohexane degrading bacterium, Sphingomonas paucimobilis UT26

Keisuke Miyauchi, Masao Fukuda, Masataka Tsuda, Masamichi Takagi, Yuji Nagata

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Tn5-derived mutants of the γ-hexachlorocyclohexane-degrading bacterium Sphingomonas paucimobilis UT26 were genetically characterized, and an endogenous insertion sequence (IS) which belongs to the IS1380 family was identified. The IS, earned ISsp1, existed as multi copies in UT26, and its transposition appeared to be activated during the process of Tn5-mutagenesis. It was found that transposon mutagenesis can cause endogenous mutations.

Original languageEnglish
Pages (from-to)216-219
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume69
Issue number1
DOIs
Publication statusPublished - 2005 Jan

Keywords

  • Biodegradation
  • Insertion sequence
  • Sphingomonas
  • Tn5 mutagenesis
  • γ-hexachlorocyclohexane

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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