Identification of human low-density lipoprotein receptor as a novel target gene regulated by liver X receptor alpha

Kenji Ishimoto; Keisuke Tachibana; Mikako Sumitomo; Shiho Omote; Ikuko Hanano; Daisuke Yamasaki; Yuichiro Watanabe; Toshiya Tanaka; Takao Hamakubo; Juro Sakai; Tatsuhiko Kodama; Takefumi Doi

doi:10.1016/j.febslet.2006.08.010

Identification of human low-density lipoprotein receptor as a novel target gene regulated by liver X receptor alpha

Kenji Ishimoto, Keisuke Tachibana, Mikako Sumitomo, Shiho Omote, Ikuko Hanano, Daisuke Yamasaki, Yuichiro Watanabe, Toshiya Tanaka, Takao Hamakubo, Juro Sakai, Tatsuhiko Kodama, Takefumi Doi

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

Abstract

Liver X receptor alpha (LXRα) is a member of the nuclear receptor superfamily that is activated by oxysterols, and plays a pivotal role in regulating the metabolism, transport and uptake of cholesterol. Here, we demonstrate that LXRα also regulates the low-density lipoprotein receptor (LDLR) gene, which mediates the endocytic uptake of LDL cholesterol in the liver. An LXR agonist induced the expression of LDLR in cultured hepatoblastoma cells. Moreover, the LDLR promoter contained an LXR response element that was recognized by LXRα/RXRα (retinoid X receptor alpha) heterodimers in hepatoblastoma cells. These results suggest a novel pathway whereby LXRα might modulate cholesterol metabolism.

Original language	English
Pages (from-to)	4929-4933
Number of pages	5
Journal	FEBS Letters
Volume	580
Issue number	20
DOIs	https://doi.org/10.1016/j.febslet.2006.08.010
Publication status	Published - 2006 Sep 4
Externally published	Yes

Keywords

Cholesterol
LXR response element
Liver X receptor
Low-density lipoprotein receptor
Nuclear receptor
SREBP

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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Identification of human low-density lipoprotein receptor as a novel target gene regulated by liver X receptor alpha. / Ishimoto, Kenji; Tachibana, Keisuke; Sumitomo, Mikako; Omote, Shiho; Hanano, Ikuko; Yamasaki, Daisuke; Watanabe, Yuichiro; Tanaka, Toshiya; Hamakubo, Takao; Sakai, Juro; Kodama, Tatsuhiko; Doi, Takefumi.

In: FEBS Letters, Vol. 580, No. 20, 04.09.2006, p. 4929-4933.

Research output: Contribution to journal › Article › peer-review

Ishimoto, Kenji ; Tachibana, Keisuke ; Sumitomo, Mikako ; Omote, Shiho ; Hanano, Ikuko ; Yamasaki, Daisuke ; Watanabe, Yuichiro ; Tanaka, Toshiya ; Hamakubo, Takao ; Sakai, Juro ; Kodama, Tatsuhiko ; Doi, Takefumi. / Identification of human low-density lipoprotein receptor as a novel target gene regulated by liver X receptor alpha. In: FEBS Letters. 2006 ; Vol. 580, No. 20. pp. 4929-4933.

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abstract = "Liver X receptor alpha (LXRα) is a member of the nuclear receptor superfamily that is activated by oxysterols, and plays a pivotal role in regulating the metabolism, transport and uptake of cholesterol. Here, we demonstrate that LXRα also regulates the low-density lipoprotein receptor (LDLR) gene, which mediates the endocytic uptake of LDL cholesterol in the liver. An LXR agonist induced the expression of LDLR in cultured hepatoblastoma cells. Moreover, the LDLR promoter contained an LXR response element that was recognized by LXRα/RXRα (retinoid X receptor alpha) heterodimers in hepatoblastoma cells. These results suggest a novel pathway whereby LXRα might modulate cholesterol metabolism.",

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