Identification of human dehydrodolichyl diphosphate synthase gene

Shota Endo, Yuan Wei Zhang, Seiji Takahashi, Tanetoshi Koyama

Research output: Contribution to journalArticlepeer-review

47 Citations (Scopus)

Abstract

We isolated a cDNA encoding human dehydrodolichyl diphosphate (Dedol-PP) synthase and expressed the gene in a yeast mutant strain SNH23-7D, which is deficient in Dedol-PP synthase activity. The identity of the cloned enzyme was confirmed by functional complementation of SNH23-7D strain together with in vitro Dedol-PP synthase activity assay. Northern blot analysis indicated that testis and kidney expressed Dedol-PP synthase mRNA at high levels.

Original languageEnglish
Pages (from-to)291-295
Number of pages5
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1625
Issue number3
DOIs
Publication statusPublished - 2003 Feb 20

Keywords

  • Cis-Prenyl chain elongation
  • Dehydrodolichyl diphosphate synthase
  • Dolichol biosynthesis
  • Glycosyl carrier lipid

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

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