Identification of histamine-production-increasing factor produced by stimulated RBL-2H3 rat basophilic leukemia cells as granulocyte-macrophage colony-stimulating factor

Muneshige Shiraishi, Noriyasu Hirasawa, Suetsugu Mue, Kazuo Ohuchi

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

When RBL-2H3 rat basophilic leukemia cells were stimulated by antigen or the Ca2+ ionophore A23187, the activity to increase histamine production by rat bone marrow cells in the conditioned medium increased time-dependently. To characterize the histamine-production-increasing factor (HPIF) produced by RBL-2H3 cells, the conditioned medium was collected 8 h after stimulation by A23187, and the factor was purified by three-step chromatography, the specific activity being increased by 9000-fold. The partial amino acid sequence of the peptide obtained by S. aureus V8 protease digestion was identical to the internal amino acid sequence of rat granulocyte-macrophage colony-stimulating factor (GM-CSF). In addition, GM-CSF mRNA levels in RBL-2H3 cells were increased by A23187 with a peak at 4 h. Furthermore, recombinant rat GM-CSF increased histamine production by rat bone marrow cells. These findings suggested that HPIF produced by the stimulated RBL-2H3 cells is GM-CSF. Possible significant roles of HPIF at the late phase of allergic inflammation are discussed. Copyright (C) 1998 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)273-280
Number of pages8
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1403
Issue number3
DOIs
Publication statusPublished - 1998 Jul 24

Keywords

  • Bone marrow cell
  • Granulocyte-macrophage colony-stimulating factor
  • Histamine-production-increasing factor
  • Rat basophilic leukemia-2H3 cell

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Identification of histamine-production-increasing factor produced by stimulated RBL-2H3 rat basophilic leukemia cells as granulocyte-macrophage colony-stimulating factor'. Together they form a unique fingerprint.

Cite this