Identification of glucosylceramides containing sphingatrienine in maize and rice using ion trap mass spectrometry

Tatsuya Sugawara, Jingjing Duan, Kazuhiko Aida, Tsuyoshi Tsuduki, Takashi Hirata

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)

Abstract

We characterized the glucosylceramide moieties from maize and rice using liquid chromatography-ion trap mass spectrometry. Glucosylceramides containing 4,8-sphingadienine (d18:2) acylated to hydroxy-fatty acids were detected as the predominant molecules both in maize and in rice. In addition, 4-hydroxy-8-sphingenine (t18:1) and sphingatrienine (d18:3) were found in maize and rice glucosylceramides, and in the case of rice, sphingenine (d18:1) was also detected. Glucosylceramides containing d18:3 were acylated to hydroxyl fatty acids (16-24 carbon atoms). Our results indicate the presence of the triene type of sphingoid base in higher plants.

Original languageEnglish
Pages (from-to)451-455
Number of pages5
JournalLipids
Volume45
Issue number5
DOIs
Publication statusPublished - 2010 May 1

Keywords

  • Glycosphingolipids
  • HPLC (High performance liquid chromatography)
  • Mass spectrometry (MS)
  • Plant lipids
  • Sphingoid bases

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry
  • Cell Biology

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