Identification of genetic alterations in extramammary Paget disease using whole exome analysis

Yukiko Kiniwa, Jun Yasuda, Sakae Saito, Rumiko Saito, Ikuko N. Motoike, Inaho Danjoh, Kengo Kinoshita, Nobuo Fuse, Masayuki Yamamoto, Ryuhei Okuyama

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)


Background: Extramammary Paget disease (EMPD)is a rare cutaneous malignant neoplasm, and the genomic alterations underlying its pathogenesis are unknown. Objective: To identify tumor-specific genomic alterations in EMPD. Methods: Exome analysis was performed in specimens from three EMPD patients, and target amplicon sequencing was done for genes frequently mutated in other adenocarcinomas. Results: Exome analysis revealed recurrent somatic mutations in several genes, includingTP53, PIK3CA, and ERBB2. We identified additional candidate exons by searching the COSMIC database for exons that are frequently mutated in other adenocarcinomas. We obtained 19 exons in 12 genes as candidate exons, and performed target amplicon sequencing in samples obtained from EMPD patients. New somatic mutations in the TP53 gene were identified in six EMPD patients. Single nucleotide polymorphism analysis revealed multiple chromosomal alterations in three EMPD specimens, and two specimens exhibited amplification of chromosome 12p13 and losses of 3p21–24, 7q22 and 13q12–21. Conclusion: Our comprehensive genetic analysis identified novel genomic alterations, and will inform treatment options for EMPD.

Original languageEnglish
Pages (from-to)229-235
Number of pages7
JournalJournal of dermatological science
Issue number1
Publication statusPublished - 2019 Apr


  • Chromosomal copy number alteration
  • ERBB2
  • Exome
  • Extramammary Paget disease
  • PIK3CA
  • TP53

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology


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