Identification of genes expressed in the shoot apex of Brassica campestris during floral transition

Hiroyasu Kitashiba, Takayoshi Iwai, Kinya Toriyama, Masao Watanabe, Kokichi Hinata

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

The vegetative-to-floral transition of Brassica campestris cv. Osome was induced by vernalization. Poly(A)+RNA was isolated from the transition shoot apex after 6 weeks of vernalization, the floral apex after 12 weeks of vernalization and the expanded leaves just before vernalization, and cDNAs were synthesized. These cDNAs were used for subtraction and differential screening to select cDNA preferentially present in the transition and floral apices. Nucleotide sequences of the resulting 14 cDNA clones were determined, and northern blot analysis was carried out on six cDNAs. Two cDNA clones which did not show significant similarity to known genes were shown to be preferentially expressed in the floral apex.

Original languageEnglish
Pages (from-to)186-188
Number of pages3
JournalSexual Plant Reproduction
Volume9
Issue number3
DOIs
Publication statusPublished - 1996 Jan 1

Keywords

  • Brassica campestris
  • Floral transition
  • Shoot apex
  • Vernalization
  • cDNA cloning

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

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