Identification of five phospho-β-glycosidases from Lactobacillus gasseri ATCC33323T cultured in lactose medium

Seiji Nagaoka; Hiroyuki Honda; Satoru Ohshima; Yasushi Kawai; Haruki Kitazawa; Yoshio Tateno; Yukiko Yamazaki; Tadao Saito

doi:10.1271/bbb.80089

Identification of five phospho-β-glycosidases from Lactobacillus gasseri ATCC33323^T cultured in lactose medium

Seiji Nagaoka, Hiroyuki Honda, Satoru Ohshima, Yasushi Kawai, Haruki Kitazawa, Yoshio Tateno, Yukiko Yamazaki, Tadao Saito

AGR - Bioscience and Biotechnology for Future Bioindustries

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Lactobacillus gasseri ATCC33323^T has seven putative phospho-β-glycosidase genes. Using column chromatography, we found that this strain cultured in lactose medium expresses five phospho-β- glycosidases (LacG1, LacG2, Pbg1, Pbg2, and Pbg3), where these gene expressions can be suppressed by glucose. To our knowledge, this is the first report indicating that five glycosidases are induced from a single bacterial strain using a single carbon source, lactose.

Original language	English
Pages (from-to)	1954-1957
Number of pages	4
Journal	Bioscience, Biotechnology and Biochemistry
Volume	72
Issue number	7
DOIs	https://doi.org/10.1271/bbb.80089
Publication status	Published - 2008 Jul 30

Keywords

Lactobacillus gasseri
Lactose
Phospho-β- glucosidase
Phospho-β-galactosidase

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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Identification of five phospho-β-glycosidases from Lactobacillus gasseri ATCC33323^T cultured in lactose medium. / Nagaoka, Seiji; Honda, Hiroyuki; Ohshima, Satoru; Kawai, Yasushi; Kitazawa, Haruki; Tateno, Yoshio; Yamazaki, Yukiko; Saito, Tadao.

In: Bioscience, Biotechnology and Biochemistry, Vol. 72, No. 7, 30.07.2008, p. 1954-1957.

Research output: Contribution to journal › Article › peer-review

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abstract = "Lactobacillus gasseri ATCC33323T has seven putative phospho-β-glycosidase genes. Using column chromatography, we found that this strain cultured in lactose medium expresses five phospho-β- glycosidases (LacG1, LacG2, Pbg1, Pbg2, and Pbg3), where these gene expressions can be suppressed by glucose. To our knowledge, this is the first report indicating that five glycosidases are induced from a single bacterial strain using a single carbon source, lactose.",

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AU - Kawai, Yasushi

AU - Kitazawa, Haruki

AU - Tateno, Yoshio

AU - Yamazaki, Yukiko

AU - Saito, Tadao

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