Identification of five phospho-β-glycosidases from Lactobacillus gasseri ATCC33323T cultured in lactose medium

Seiji Nagaoka; Hiroyuki Honda; Satoru Ohshima; Yasushi Kawai; Haruki Kitazawa; Yoshio Tateno; Yukiko Yamazaki; Tadao Saito

doi:10.1271/bbb.80089

Identification of five phospho-β-glycosidases from Lactobacillus gasseri ATCC33323^T cultured in lactose medium

Seiji Nagaoka, Hiroyuki Honda, Satoru Ohshima, Yasushi Kawai, Haruki Kitazawa, Yoshio Tateno, Yukiko Yamazaki, Tadao Saito

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Lactobacillus gasseri ATCC33323^T has seven putative phospho-β-glycosidase genes. Using column chromatography, we found that this strain cultured in lactose medium expresses five phospho-β- glycosidases (LacG1, LacG2, Pbg1, Pbg2, and Pbg3), where these gene expressions can be suppressed by glucose. To our knowledge, this is the first report indicating that five glycosidases are induced from a single bacterial strain using a single carbon source, lactose.

Original language	English
Pages (from-to)	1954-1957
Number of pages	4
Journal	Bioscience, Biotechnology and Biochemistry
Volume	72
Issue number	7
DOIs	https://doi.org/10.1271/bbb.80089
Publication status	Published - 2008

Keywords

Lactobacillus gasseri
Lactose
Phospho-β- glucosidase
Phospho-β-galactosidase

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

Access to Document

10.1271/bbb.80089

Cite this

@article{2bb97071bf984f028c728bbfcc2a005d,

title = "Identification of five phospho-β-glycosidases from Lactobacillus gasseri ATCC33323T cultured in lactose medium",

abstract = "Lactobacillus gasseri ATCC33323T has seven putative phospho-β-glycosidase genes. Using column chromatography, we found that this strain cultured in lactose medium expresses five phospho-β- glycosidases (LacG1, LacG2, Pbg1, Pbg2, and Pbg3), where these gene expressions can be suppressed by glucose. To our knowledge, this is the first report indicating that five glycosidases are induced from a single bacterial strain using a single carbon source, lactose.",

keywords = "Lactobacillus gasseri, Lactose, Phospho-β- glucosidase, Phospho-β-galactosidase",

author = "Seiji Nagaoka and Hiroyuki Honda and Satoru Ohshima and Yasushi Kawai and Haruki Kitazawa and Yoshio Tateno and Yukiko Yamazaki and Tadao Saito",

note = "Funding Information: This work was partially supported by a grant-in-aid for Scientific Research (B) (no. 16380179) from the Japan Society for the Promotion of Science (JSPS), the Food Science Institute Foundation (Ryoshoku Kenkukai), and the National Institute of Genetics (NIG) Cooperative Research Program (2006-B44) (to TS). The authors thank Mr. Kenichi Hojo for critical reading of the manuscript.",

year = "2008",

doi = "10.1271/bbb.80089",

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pages = "1954--1957",

journal = "Bioscience, Biotechnology and Biochemistry",

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publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",

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T1 - Identification of five phospho-β-glycosidases from Lactobacillus gasseri ATCC33323T cultured in lactose medium

AU - Nagaoka, Seiji

AU - Honda, Hiroyuki

AU - Ohshima, Satoru

AU - Kawai, Yasushi

AU - Kitazawa, Haruki

AU - Tateno, Yoshio

AU - Yamazaki, Yukiko

AU - Saito, Tadao

N1 - Funding Information: This work was partially supported by a grant-in-aid for Scientific Research (B) (no. 16380179) from the Japan Society for the Promotion of Science (JSPS), the Food Science Institute Foundation (Ryoshoku Kenkukai), and the National Institute of Genetics (NIG) Cooperative Research Program (2006-B44) (to TS). The authors thank Mr. Kenichi Hojo for critical reading of the manuscript.

PY - 2008

Y1 - 2008

N2 - Lactobacillus gasseri ATCC33323T has seven putative phospho-β-glycosidase genes. Using column chromatography, we found that this strain cultured in lactose medium expresses five phospho-β- glycosidases (LacG1, LacG2, Pbg1, Pbg2, and Pbg3), where these gene expressions can be suppressed by glucose. To our knowledge, this is the first report indicating that five glycosidases are induced from a single bacterial strain using a single carbon source, lactose.

AB - Lactobacillus gasseri ATCC33323T has seven putative phospho-β-glycosidase genes. Using column chromatography, we found that this strain cultured in lactose medium expresses five phospho-β- glycosidases (LacG1, LacG2, Pbg1, Pbg2, and Pbg3), where these gene expressions can be suppressed by glucose. To our knowledge, this is the first report indicating that five glycosidases are induced from a single bacterial strain using a single carbon source, lactose.

KW - Lactobacillus gasseri

KW - Lactose

KW - Phospho-β- glucosidase

KW - Phospho-β-galactosidase

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