Identification of fish species in dried fish products by immunostaining using anti-myosin light chain antiserum

Y. Ochiai, S. Watabe

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

In order to immunologically detect fish species used in boiled and dried fish products (dried fish sticks), the availability of anti-serum against myosin light chains was examined. After dissolving the samples in the presence of 8 M urea and 1% sodium dodecyl sulfate (SDS), the solubilized matters were applied to SDS-polyacrylamide gel electrophoresis and electroblotted onto PVDF membranes. Subsequently, protein bands concerned were stained using anti-myosin light chain (alkali light chain 1) rabbit anti-serum and horseradish peroxidase-conjugated anti-rabbit immunoglobulin. Most of the fish species could be identified by finger-printing of immunostained patterns even after drastic processing, even though the protein staining patterns were not clear enough for species identification.

Original languageEnglish
Pages (from-to)1029-1035
Number of pages7
JournalFood Research International
Volume36
Issue number9-10
DOIs
Publication statusPublished - 2003
Externally publishedYes

Keywords

  • Antiserum
  • Fish stick
  • Immunodetection
  • Myosin light chain
  • Species authentification

ASJC Scopus subject areas

  • Food Science

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