Identification of FAP locus genes from chromosome 5q21

Kenneth W. Kinzler, Mef C. Nilbert, Li Kuo Su, Bert Vogelstein, Tracy M. Bryan, Daniel B. Levy, Kelly J. Smith, Antonette C. Preisinger, Philip Hedge, Douglas McKechnie, Rachel Finniear, Alex Markham, John Groffen, Mark S. Boguski, Stephen F. Altschul, Akira Horii, Hiroshi Ando, Yasuo Miyoshi, Yoshio Miki, Isamu NishishoYusuke Nakamura

Research output: Contribution to journalArticlepeer-review

1897 Citations (Scopus)

Abstract

Recent studies suggest that one or more genes on chromosome 5q21 are important for the development of colorectal cancers, particularly those associated with familial adenomatous polyposis (FAP). To facilitate the identification of genes from this locus, a portion of the region that is tightly linked to FAP was cloned. Six contiguous stretches of sequence (contigs) containing approximately 5.5 Mb of DNA were isolated. Subclones from these contigs were used to identify and position six genes, all of which were expressed in normal colonic mucosa. Two of these genes (APC and MCC) are likely to contribute to colorectal tumorigenesis. The MCC gene had previously been identified by virtue of its mutation in human colorectal tumors. The APC gene was identified in a contig initiated from the MCC gene and was found to encode an unusually large protein. These two closely spaced genes encode proteins predicted to contain coiled-coil regions. Both genes were also expressed in a wide variety of tissues. Further studies of MCC and APC and their potential interaction should prove useful for understanding colorectal neoplasia.

Original languageEnglish
Pages (from-to)661-665
Number of pages5
JournalScience
Volume253
Issue number5020
DOIs
Publication statusPublished - 1991

ASJC Scopus subject areas

  • General

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