Identification of F-box proteins that are involved in resistance to methylmercury in Saccharomyces cerevisiae

Giwook Hwang, Yosuke Ishida, Akira Naganuma

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

We searched for F-box proteins that might be related to the mechanism that protects Saccharomyces cerevisiae against the toxic effects of methylmercury. We found that overexpression of Hrt3 and of Ylr224w rendered yeast cells resistant to methylmercury. Yeast cells that overexpressed Hrt3 and Ylr224w were barely resistant to methylmercury in the presence of a proteasome inhibitor. Our results suggest the existence of some protein(s) that enhances the toxicity of methylmercury in yeast cells and, also, that overexpression of Hrt3 or Ylr224w can confer resistance to methylmercury by enhancing the polyubiquitination of this protein(s) and its degradation in proteasomes.

Original languageEnglish
Pages (from-to)6813-6818
Number of pages6
JournalFEBS Letters
Volume580
Issue number30
DOIs
Publication statusPublished - 2006 Dec 22

Keywords

  • F-box protein
  • Methylmercury
  • Resistance
  • Ubiquitin
  • Yeast

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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