Identification of dislocations in large tetragonal hen egg-white lysozyme crystals by synchrotron white-beam topography

M. Tachibana, H. Koizumi, K. Izumi, K. Kajiwara, K. Kojima

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Large tetragonal hen egg-white (HEW) lysozyme crystals have been grown by a salt concentration-gradient method. The grown crystals, of thicknesses greater than 1.5 mm, were observed by means of X-ray topography using white-beam synchrotron radiation. Line contrasts clearly appeared on the Laue topographs. Extinction of the line images was observed in specific reflections. These results mean that the observed lines correspond to dislocation images. From the extinction criterion it is shown that the predominant dislocations are of screw character with 〈110〉 Burgers vectors. In addition, dislocation loops with [001] Burgers vectors have been found in a tetragonal HEW lysozyme crystal including some cracks. These results are discussed in the light of dislocation elastic energy and slip systems in the crystals.

Original languageEnglish
Pages (from-to)416-420
Number of pages5
JournalJournal of Synchrotron Radiation
Volume10
Issue number6
DOIs
Publication statusPublished - 2003 Nov 1

Keywords

  • Crystal defects
  • Dislocations
  • Lysozyme
  • Protein crystals
  • X-ray topography

ASJC Scopus subject areas

  • Radiation
  • Nuclear and High Energy Physics
  • Instrumentation

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