Identification of cDNA coding for a homologue to mammalian leptin from pufferfish, Takifugu rubripes

Tadahide Kurokawa, Susumu Uji, Tohru Suzuki

Research output: Contribution to journalArticlepeer-review

171 Citations (Scopus)

Abstract

We identified cDNA coding for a homologue to mammalian leptin in puffer, Takifugu rubripes, using genomic synteny around the human leptin gene. In addition to significant sequence homologies, the puffer leptin (pLEP) displays characteristic structural features in common with mammalian leptin. The pLEP mRNA was expressed mostly in the liver that contained abundant lipids. In addition, homologues to pLEP were found in the databanks for three fish species (salmon, medaka, and Tetraodon) and two amphibians (salamander and Xenopus). The phylogenetic analysis shows rapid rates of molecular divergence among leptins from different vertebrate classes, but not between mammals and avians.

Original languageEnglish
Pages (from-to)745-750
Number of pages6
JournalPeptides
Volume26
Issue number5
DOIs
Publication statusPublished - 2005 May

Keywords

  • Amphibian
  • Fish
  • Leptin
  • Takifugu rubripes
  • cDNA

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

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