Identification of Candida species from human immunodeficiency virus-infected patients in Ethiopia by combination of CHROMagar, Tobacco agar and PCR of amplified internally transcribed rRNA spacer region

Hiroshi Isogai, Andargachew Mulu, Erimias Diro, Henok Tekleselassie, Afework Kassu, Koichi Kimura, Takeshi Nishikawa, Emiko Isogai

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

The purpose of this study was to determine phenotypic and genotypic identification of Candida species from HIV infected patients in Ethiopia. Oral rinses from 13 human immunodeficiency virus (HIV) positive patients were inoculated on CHROMagar. Colonies were then sub cultured on To bacco agar. DNA was extracted from 71 colonies and amplified by PCR targeting the conserved regions of 18S, 5.8S, and 28S rRNAs. The amplified DNA samples were analyzed their sequence and a part of them were examined by restriction fragment length polymorphism (RFLP). Oral Candida colonization was detected in all of patients with or without oral candidiasis. The number of Candida species on the CHRO Magar was more than 1000 CFU/ml in 12 of 13 patients. We showed that phenotypic and genotypic identification process was important to determine the species. These results indicated that HIV-positive patients have high risk to C. albicans and the other Candida speceies infection in Ethipia.

Original languageEnglish
Pages (from-to)2-8
Number of pages7
JournalJournal of Applied Research
Volume10
Issue number1
Publication statusPublished - 2010 Jul 9

Keywords

  • CHROMagar
  • Candida
  • Ethiopia
  • Human immunodeficiency virus infection
  • Tobacco agar
  • rRNA spacer region

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)

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