Identification of bovine invariant chain (Ii) gene by nucleotide sequencing

Masashi Niimi, Yutaka Nakai, Yoko Aida

    Research output: Contribution to journalArticlepeer-review

    5 Citations (Scopus)

    Abstract

    Four overlapping cDNA clones encoding the bovine invariant chain (Ii) were isolated and characterized. The bovine Ii cDNA clone, NI3 with a 1381-bp insert, encoded a translated product of 204 amino acids. The amino acid sequences deduced from this clone revealed that the bovine Ii gene is more closely related to human Ii gene than to genes for rodents, such as mouse and rat, but lacked 10 amino acids of the 3' end of the extracellular domain comparable to those in proteins from other species. Of interest is that the class-II-associated invariant chain peptide (CLIP) segment is present in the protein encoded by N13, and this region exhibits a high degree of overall similarity to sequences encoded by human, mouse and rat, suggesting that bovine CLIP may have the biological function of CLIP binding to MHC class II molecules. Thus, it seems likely that this cDNA clone encodes a functional product which might perform an important function in MHC class II antigen presentation, as previously established in studies in mouse and man.

    Original languageEnglish
    Pages (from-to)7-12
    Number of pages6
    JournalBiochemical and biophysical research communications
    Volume222
    Issue number1
    DOIs
    Publication statusPublished - 1996 May 6

    ASJC Scopus subject areas

    • Biophysics
    • Biochemistry
    • Molecular Biology
    • Cell Biology

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