TY - JOUR
T1 - Identification of bovine invariant chain (Ii) gene by nucleotide sequencing
AU - Niimi, Masashi
AU - Nakai, Yutaka
AU - Aida, Yoko
N1 - Funding Information:
This study was supported by Special Coordination Funds from the Science and Technology Agency of the Japanese Government, and funding from the Livestock Improvement Association of Japan Inc.
PY - 1996/5/6
Y1 - 1996/5/6
N2 - Four overlapping cDNA clones encoding the bovine invariant chain (Ii) were isolated and characterized. The bovine Ii cDNA clone, NI3 with a 1381-bp insert, encoded a translated product of 204 amino acids. The amino acid sequences deduced from this clone revealed that the bovine Ii gene is more closely related to human Ii gene than to genes for rodents, such as mouse and rat, but lacked 10 amino acids of the 3' end of the extracellular domain comparable to those in proteins from other species. Of interest is that the class-II-associated invariant chain peptide (CLIP) segment is present in the protein encoded by N13, and this region exhibits a high degree of overall similarity to sequences encoded by human, mouse and rat, suggesting that bovine CLIP may have the biological function of CLIP binding to MHC class II molecules. Thus, it seems likely that this cDNA clone encodes a functional product which might perform an important function in MHC class II antigen presentation, as previously established in studies in mouse and man.
AB - Four overlapping cDNA clones encoding the bovine invariant chain (Ii) were isolated and characterized. The bovine Ii cDNA clone, NI3 with a 1381-bp insert, encoded a translated product of 204 amino acids. The amino acid sequences deduced from this clone revealed that the bovine Ii gene is more closely related to human Ii gene than to genes for rodents, such as mouse and rat, but lacked 10 amino acids of the 3' end of the extracellular domain comparable to those in proteins from other species. Of interest is that the class-II-associated invariant chain peptide (CLIP) segment is present in the protein encoded by N13, and this region exhibits a high degree of overall similarity to sequences encoded by human, mouse and rat, suggesting that bovine CLIP may have the biological function of CLIP binding to MHC class II molecules. Thus, it seems likely that this cDNA clone encodes a functional product which might perform an important function in MHC class II antigen presentation, as previously established in studies in mouse and man.
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U2 - 10.1006/bbrc.1996.0689
DO - 10.1006/bbrc.1996.0689
M3 - Article
C2 - 8630076
AN - SCOPUS:0029973009
VL - 222
SP - 7
EP - 12
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -