TY - JOUR
T1 - Identification of bovine dendritic cell phenotype from bovine peripheral blood
AU - Miyazawa, Kohtaro
AU - Aso, Hisashi
AU - Honda, Masashi
AU - Kido, Taketomo
AU - Minashima, Takeshi
AU - Kanaya, Takashi
AU - Watanabe, Kouichi
AU - Ohwada, Shyuichi
AU - Rose, Michael T.
AU - Yamaguchi, Takahiro
PY - 2006/8/1
Y1 - 2006/8/1
N2 - Dendritic cells (DCs) are professional antigen presenting cells, which initiate primary immune responses and also play an important role in the generation of peripheral tolerance. There is no reliable method established for the isolation of bovine peripheral blood DCs, and furthermore, the phenotypes and the functions of bovine DCs are still not fully clear. In the present study, we have attempted to identify bovine peripheral blood DCs by negative-selection. In bovine peripheral blood mononuclear cells (PBMC), we have newly characterized the phenotype of DCs, which is CD11c+/CD172a+. These cells display features of myeloid type DCs. In the thymic medulla, CD11c+/CD172a+ cells were also present and CD1+/CD172a+ cells were additionally detected as a population of DCs. The data suggest that one of the bovine DCs phenotypes from PBMC is derived from myeloid lineages lacking a CD1 molecule, which then drift to several tissues, and that they then may express a CD1 molecule upon their functional differentiation.
AB - Dendritic cells (DCs) are professional antigen presenting cells, which initiate primary immune responses and also play an important role in the generation of peripheral tolerance. There is no reliable method established for the isolation of bovine peripheral blood DCs, and furthermore, the phenotypes and the functions of bovine DCs are still not fully clear. In the present study, we have attempted to identify bovine peripheral blood DCs by negative-selection. In bovine peripheral blood mononuclear cells (PBMC), we have newly characterized the phenotype of DCs, which is CD11c+/CD172a+. These cells display features of myeloid type DCs. In the thymic medulla, CD11c+/CD172a+ cells were also present and CD1+/CD172a+ cells were additionally detected as a population of DCs. The data suggest that one of the bovine DCs phenotypes from PBMC is derived from myeloid lineages lacking a CD1 molecule, which then drift to several tissues, and that they then may express a CD1 molecule upon their functional differentiation.
KW - Bovine
KW - Dendritic cells
KW - Negative-selection
KW - PBMC
KW - Phenotypes
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U2 - 10.1016/j.rvsc.2005.09.003
DO - 10.1016/j.rvsc.2005.09.003
M3 - Article
C2 - 16253299
AN - SCOPUS:33644655815
VL - 81
SP - 40
EP - 45
JO - Research in Veterinary Science
JF - Research in Veterinary Science
SN - 0034-5288
IS - 1
ER -