Identification of bovine dendritic cell phenotype from bovine peripheral blood

Kohtaro Miyazawa, Hisashi Aso, Masashi Honda, Taketomo Kido, Takeshi Minashima, Takashi Kanaya, Kouichi Watanabe, Shyuichi Ohwada, Michael T. Rose, Takahiro Yamaguchi

    Research output: Contribution to journalArticlepeer-review

    29 Citations (Scopus)

    Abstract

    Dendritic cells (DCs) are professional antigen presenting cells, which initiate primary immune responses and also play an important role in the generation of peripheral tolerance. There is no reliable method established for the isolation of bovine peripheral blood DCs, and furthermore, the phenotypes and the functions of bovine DCs are still not fully clear. In the present study, we have attempted to identify bovine peripheral blood DCs by negative-selection. In bovine peripheral blood mononuclear cells (PBMC), we have newly characterized the phenotype of DCs, which is CD11c+/CD172a+. These cells display features of myeloid type DCs. In the thymic medulla, CD11c+/CD172a+ cells were also present and CD1+/CD172a+ cells were additionally detected as a population of DCs. The data suggest that one of the bovine DCs phenotypes from PBMC is derived from myeloid lineages lacking a CD1 molecule, which then drift to several tissues, and that they then may express a CD1 molecule upon their functional differentiation.

    Original languageEnglish
    Pages (from-to)40-45
    Number of pages6
    JournalResearch in Veterinary Science
    Volume81
    Issue number1
    DOIs
    Publication statusPublished - 2006 Aug

    Keywords

    • Bovine
    • Dendritic cells
    • Negative-selection
    • PBMC
    • Phenotypes

    ASJC Scopus subject areas

    • veterinary(all)

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