Identification of binding proteins for tsc22d1 family proteins using mass spectrometry

Ryouta Kamimura, Daisuke Uchida, Shin Ichiro Kanno, Ryo Shiraishi, Toshiki Hyodo, Yuta Sawatani, Michiko Shimura, Tomonori Hasegawa, Maki Tsubura-Okubo, Erika Yaguchi, Yuske Komiyama, Chonji Fukumoto, Sayaka Izumi, Atsushi Fujita, Takahiro Wakui, Hitoshi Kawamata

Research output: Contribution to journalArticlepeer-review

Abstract

TSC-22 (TGF-β stimulated clone-22) has been reported to induce differentiation, growth inhibition, and apoptosis in various cells. TSC-22 is a member of a family in which many proteins are produced from four different family genes. TSC-22 (corresponding to TSC22D1-2) is composed of 144 amino acids translated from a short variant mRNA of the TSC22D1 gene. In this study, we attempted to determine the intracellular localizations of the TSC22D1 family proteins (TSC22D1-1, TSC-22 (TSC22D1-2), and TSC22(86) (TSC22D1-3)) and identify the binding proteins for TSC22D1 family proteins by mass spectrometry. We determined that TSC22D1-1 was mostly localized in the nucleus, TSC-22 (TSC22D1-2) was localized in the cytoplasm, mainly in the mitochondria and trans-located from the cytoplasm to the nucleus after DNA damage, and TSC22(86) (TSC22D1-3) was localized in both the cytoplasm and nucleus. We identified multiple candidates of binding proteins for TSC22D1 family proteins in in vitro pull-down assays and in vivo binding assays. Histone H1 bound to TSC-22 (TSC22D1-2) or TSC22(86) (TSC22D1-3) in the nucleus. Guanine nucleotide-bind-ing protein-like 3 (GNL3), which is also known as nucleostemin, bound to TSC-22 (TSC22D1-2) in the nucleus. Further investigation of the interaction of the candidate binding proteins with TSC22D1 family proteins would clarify the biological roles of TSC22D1 family proteins in several cell systems.

Original languageEnglish
Article number10913
JournalInternational journal of molecular sciences
Volume22
Issue number20
DOIs
Publication statusPublished - 2021 Oct 1

Keywords

  • Binding protein
  • Differentiation-inducing therapy
  • Guanine nucleotide-binding protein-like 3 (GNL3)
  • Histone H1
  • Mass spectrometry
  • Molecular-targeted therapy
  • TSC22D1 family proteins

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Fingerprint

Dive into the research topics of 'Identification of binding proteins for tsc22d1 family proteins using mass spectrometry'. Together they form a unique fingerprint.

Cite this