Identification of Bach2 as a B-cell-specific partner for small Maf proteins that negatively regulate the immunoglobulin heavy chain gene 3' enhancer

Akihiko Muto, Hideto Hoshino, Linda Madisen, Nobuaki Yanai, Masuo Obinata, Hajime Karasuyama, Norio Hayashi, Hiromitsu Nakauchi, Masayuki Yamamoto, Mark Groudine, Kazuhiko Igarashi

Research output: Contribution to journalArticlepeer-review

145 Citations (Scopus)

Abstract

Maf family transcription factors are important regulators in various differentiation systems. Putative Maf recognition elements (MAREs) are found in the 3' enhancer region of the immunoglobulin heavy chain (IgH) gene. These elements are bound in B-cell extracts by a heterodimeric protein complex containing both Bach2 and a small Maf protein. Analysis of normal hematopoietic cells revealed that Bach2 is specifically expressed in B cells. Bach2 is abundantly expressed in the early stages of B-cell differentiation and turned off in terminally differentiated cells. Bach2 acts together with MafK as a negative effector of the IgH 3' enhancer and binds to the co-repressor SMRT (silencing mediator of retinoid and thyroid receptor). Hence the Bach2-small-Maf heterodimer may represent the first example of a B-cell lineage, and of a developmental stage-restricted negative effector of the MARE in the IgH 3' enhancer region.

Original languageEnglish
Pages (from-to)5734-5743
Number of pages10
JournalEMBO Journal
Volume17
Issue number19
DOIs
Publication statusPublished - 1998 Oct 1

Keywords

  • B cell
  • Immunoglobulin
  • Maf
  • Transcription factor

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Fingerprint Dive into the research topics of 'Identification of Bach2 as a B-cell-specific partner for small Maf proteins that negatively regulate the immunoglobulin heavy chain gene 3' enhancer'. Together they form a unique fingerprint.

Cite this