Identification of anther-specific genes in a cruciferous model plant, Arabidopsis thaliana, by using a combination of Arabidopsis macroarray and mRNA derived from Brassica oleracea

Masayuki Amagai, Tohru Ariizumi, Makoto Endo, Katsunori Hatakeyama, Chikara Kuwata, Daisuke Shibata, Kinya Toriyama, Masao Watanabe

Research output: Contribution to journalArticlepeer-review

42 Citations (Scopus)

Abstract

Arabidopsis thaliana, a member of the Brassicaceae, is a model plant whose genome was the first higher plant genome to be sequenced. Because of the small size of the flowers, it is difficult to dissect and separate reproductive organs (anthers and pistils) at different developmental stages in A. thaliana. In order to perform genome-wide identification of anther-specific genes in A. thaliana, an Arabidopsis cDNA macroarray was hybridized to cDNA derived from anthers and pistils of another crucifer, Brassica oleracea. After scanning the signal intensity for each clone, and cluster analysis, 52 anther-specific genes were identified. These clones contained several anther-specific genes that have already been characterized, as well as novel anther-specific genes. In RT-PCR analysis with mRNA of A. thaliana and B. oleracea, the expression pattern of one-third of the clones was similar to that determined by cDNA macroarray. This system of heterologous hybridization analysis (Arabidopsis cDNA macroarray vs Brassica tissue-specific mRNA) should be applicable to other model species and their close relatives.

Original languageEnglish
Pages (from-to)213-220
Number of pages8
JournalSexual Plant Reproduction
Volume15
Issue number5
Publication statusPublished - 2003 Jan 1

Keywords

  • Anther-specific genes
  • Arabidopsis thaliana
  • Brassica oleracea
  • Heterologous hybridization
  • cDNA macroarray

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

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