Identification of annexin 1 as a PU.1 target gene in leukemia cells

Yuko Iseki, Akemi Imoto, Toshio Okazaki, Hideo Harigae, Shinichiro Takahashi

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

To identify PU.1 downstream target genes, we first established PU.1-knockdown K562 (K562PU.1KD) cells expressing reduced levels of PU.1 by stably transfected PU.1 siRNAs. From microarray analysis, we found that several genes including annexin 1 were markedly induced in K562PU.1KD cells. Annexin 1 is a calcium- and phospholipid-binding protein and increased expression leads to the constitutive activation of extracellular signal-regulated kinase (ERK). Consistent with this, we observed constitutive activation of ERK in K562PU.1KD cells. Furthermore, we revealed the mRNA expression of annexin 1 was negatively correlated with PU.1 mRNA expression in 43 primary AML specimens (R = -0.31, p < 0.042).

Original languageEnglish
Pages (from-to)1658-1663
Number of pages6
JournalLeukemia Research
Volume33
Issue number12
DOIs
Publication statusPublished - 2009 Dec

Keywords

  • AML
  • Annexin 1
  • PU.1

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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