Identification of androgen-responsive microRNAs and androgen-related genes in breast cancer

Kiichiroh Nakano, Yasuhiro Miki, Shuko Hata, Akiko Ebata, Kiyoshi Takagi, Keely M. Mcnamara, Minako Sakurai, Mariko Masuda, Hisashi Hirakawa, Takanori Ishida, Takashi Suzuki, Noriaki Ohuchi, Hironobu Sasano

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Background: Androgen receptors (ARs) are expressed in many breast cancer cells, but the mechanism of action of androgens is not as well-characterized as in other cell types. The study of microRNAs has recently provided with important insights into the biology of hormonedependent cancer. Materials and Methods: We attempted to identify microRNAs induced by dihydrotestosterone in an ARpositive cell line. We examined a possible correlation among microRNAs, target genes, and ARs in breast cancer tissues using immunohistochemistry and laser capture microscopy. Results: Our analysis demonstrated that miR-363 and its possible target IQ motif and WD repeats-1 (IQWD1) are involved in a microRNA-mRNA pathway related to the mechanism of action of androgens. Our analyses showed that a high tumor level of IQWD1 in patients with breast cancer was significantly associated with adverse clinical outcomes. Conclusion: Our findings indicated that the recruitment of IQWD1 to ARs may be a prerequisite for the growth stimulation by androgens through ARs in breast cancer cells.

Original languageEnglish
Pages (from-to)4811-4820
Number of pages10
JournalAnticancer research
Volume33
Issue number11
Publication statusPublished - 2013 Nov

Keywords

  • Androgen
  • Breast cancer
  • IQWD1
  • MicroRNA

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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