TY - JOUR
T1 - Identification of AMSH-LP containing a Jab1/MPN domain metalloenzyme motif
AU - Kikuchi, Kazu
AU - Ishii, Naoto
AU - Asao, Hironobu
AU - Sugamura, Kazuo
N1 - Funding Information:
We thank Dr. L.C. Ndhlovu for critically reading the manuscript. This work was supported in part by a Grant-in-Aid for Scientific Research (S) from the Ministry of Education, Science, Sports and Culture of Japan.
PY - 2003/7/4
Y1 - 2003/7/4
N2 - We have isolated a cDNA clone encoding a new AMSH (associated molecule with the SH3 domain of STAM) family protein, termed AMSH-like protein (AMSH-LP). AMSH-LP has similar characteristics to AMSH; both AMSH-LP and AMSH are expressed ubiquitously in various human tissues, contain a putative nuclear localization signal (NLS), an Mpr/Pad1/N-terminal (MPN) domain, and a Jab1/MPN domain metalloenzyme (JAMM) motif in their structures, and are excluded from the nucleus when lacking either the NLS or MPN domain. Moreover, we observed an enhancement of interleukin 2 (IL-2)-mediated c-myc induction in AMSH-LP-transfected cells similar to that seen in AMSH-transfected cells, suggesting a functional similarity between AMSH-LP and AMSH. However, the present study demonstrated that AMSH-LP, unlike AMSH, fails to bind to the SH3 domains of STAM1 (signal transducing adaptor molecule 1) and Grb2. These results suggest that AMSH-LP and AMSH may have different functions.
AB - We have isolated a cDNA clone encoding a new AMSH (associated molecule with the SH3 domain of STAM) family protein, termed AMSH-like protein (AMSH-LP). AMSH-LP has similar characteristics to AMSH; both AMSH-LP and AMSH are expressed ubiquitously in various human tissues, contain a putative nuclear localization signal (NLS), an Mpr/Pad1/N-terminal (MPN) domain, and a Jab1/MPN domain metalloenzyme (JAMM) motif in their structures, and are excluded from the nucleus when lacking either the NLS or MPN domain. Moreover, we observed an enhancement of interleukin 2 (IL-2)-mediated c-myc induction in AMSH-LP-transfected cells similar to that seen in AMSH-transfected cells, suggesting a functional similarity between AMSH-LP and AMSH. However, the present study demonstrated that AMSH-LP, unlike AMSH, fails to bind to the SH3 domains of STAM1 (signal transducing adaptor molecule 1) and Grb2. These results suggest that AMSH-LP and AMSH may have different functions.
KW - AMSH
KW - Grb2
KW - JAMM motif
KW - MPN domain
KW - STAMs
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U2 - 10.1016/S0006-291X(03)01009-X
DO - 10.1016/S0006-291X(03)01009-X
M3 - Article
C2 - 12810066
AN - SCOPUS:0041530206
VL - 306
SP - 637
EP - 643
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -