Identification of a subunit assembly domain in the alpha subunit of Escherichia coli RNA polymerase

Kazuhiko Igarashi, Nobuyuki Fujita, Akira Ishihama

Research output: Contribution to journalArticlepeer-review

63 Citations (Scopus)

Abstract

The α subunit of Escherichia coli DNA-dependent RNA polymerase is encoded by the rpoA gene and plays a major role in enzyme assembly. A set of C-terminal deletion mutations of the rpoA gene was constructed. The results of mixed reconstitution experiments in vitro, using the truncated α polypeptides encoded by the rpoA deletion mutants, suggest that the amino-terminal two-thirds of α subunit is sufficient for the formation of pseudo-core complexes containing both β and β′ subunits.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalJournal of Molecular Biology
Volume218
Issue number1
DOIs
Publication statusPublished - 1991 Mar 5
Externally publishedYes

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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