Identification of a receptor for Reg (regenerating gene) protein, a pancreatic β-cell regeneration factor

Seiichi Kobayashi, Takako Akiyama, Koji Nata, Michiaki Abe, Minori Tajima, Nausheen J. Shervani, Michiaki Unno, Seiki Matsuno, Hidetada Sasaki, Shin Takasawa, Hiroshi Okamoto

Research output: Contribution to journalArticlepeer-review

141 Citations (Scopus)

Abstract

Reg (regenerating gene) was isolated as a gene specifically expressed in regenerating islets (Terazono, K., Yamamoto, H., Takasawa, S., Shiga, K., Yonemura, Y., Tochino, Y., and Okamoto, H. (1988) J. Biol. Chem. 263, 2111- 2114). Rat and human Reg gene products, Reg/REG proteins, have been demonstrated to stimulate islet β-cell growth in vitro and in vivo and to ameliorate experimental diabetes. In the present study, we isolated a cDNA for the Reg protein receptor from a rat islet cDNA library. The cDNA encoded a cell surface 919-amino acid protein, and the cells into which the cDNA had been introduced bound Reg protein with high affinity. When the cDNA was introduced into RINm5F cells, a pancreatic β-cell line that shows Reg- dependent growth, the transformants exhibited significant increases in the incorporation of 5'-bromo-2'-deoxyuridine as well as in the cell numbers in response to Reg protein. A homology search revealed that the cDNA is a homologue to a human multiple exostoses-like gene, the function of which has hitherto been unknown. These results strongly suggest that the receptor is encoded by the exostoses-like gene and mediates a growth signal of Reg protein for β-cell regeneration.

Original languageEnglish
Pages (from-to)10723-10726
Number of pages4
JournalJournal of Biological Chemistry
Volume275
Issue number15
DOIs
Publication statusPublished - 2000 Apr 14

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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