Identification of a novel Cys2/His2-type zinc-finger protein as a component of a spermine-signaling pathway in tobacco

Yoshiko Mitsuya, Yoshihiro Takahashi, Yukiko Uehara, Thomas Berberich, Atsushi Miyazaki, Hideki Takahashi, Tomonobu Kusano

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

In a previous work, we identified a Cys2/His2-type zinc-finger transcription repressor, (ZFT1), that functions in a spermine-mediated signal transduction pathway in tobacco plants. Database search disclosed the presence of another Cys2/His2-type zinc-finger protein ZFP1 (accession number AAC06243) in tobacco plants. In this work, we characterized ZFP1 and investigated whether this protein is also involved in a Spm-signaling pathway. This factor showed the highest identity to petunia ZPT2-2 and higher similarity to petunia ZPT2-3, Arabidopsis STZ/ZAT10, soybean SCOF-1, red pepper CAZFP1/CaPIF1 as well as to tobacco ZFT1. ZFP1 localized to the nucleus and had a specific DNA-binding activity, supportive to be a transcription factor. Furthermore, the protein had a mild repression activity on transcription in plant cells. The expression of ZFP1, encoding ZFP1, was upregulated during tobacco mosaic virus-induced hypersensitive response. ZFP1 expression was also induced by exogenously applied spermine and its induction was repressed by inhibitors of amine oxidase/polyamine oxidase. Collectively, our data indicate that ZFP1 is a new transcription factor which functions in a spermine-signaling pathway in tobacco.

Original languageEnglish
Pages (from-to)785-793
Number of pages9
JournalJournal of Plant Physiology
Volume164
Issue number6
DOIs
Publication statusPublished - 2007 Jun 4

Keywords

  • Biotic stress
  • Polyamine catabolism
  • Spermine
  • Tobacco
  • Transcription factor

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science
  • Plant Science

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